DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS

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FATHI FARDIN; JAFARI KERMANI A.; MOULA SEYED JAVAD; POULADI A.

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Paper Information

Journal: Cell Journal (Yakhteh) Year:2008 | Volume:9 | Issue:4 (36) Page(s): 262-267

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Title

DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS

Author(s)

FATHI FARDIN | JAFARI KERMANI A. | MOULA SEYED JAVAD | POULADI A. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ2

ENDOTHELIAL CELLSQ1

EGM-2Q2

DIFFERENTIATIONQ1

Abstract

Objective: In this investigation Murine Embryonic Stem (ES) cells were differentiated into ENDOTHELIAL CELLS.Materials and Methods: Murine ES cells (CCE cell line) exposed to Alpha-MEM medium containing 10% FBS for 4 days. Then obtained Flk-1 (Flk-1: Vascular Endothelial Growth Factor Receptor 2) Positive cells were cultuted in Endothelial Growth Medium-2 (EGM-2) until the last day of experiment. Differentiated cells were evaluated by immunocytochemistry, RT-PCR and Tube Formation Assays.Results: When the ES cells cultured in collagen coated dishes containing Alpha-MEM & FBS, Flk-1 positive cells were obtained. After transfering Flk-1 positive cells into fibronectin coated dishes containing EGM2, the cells were assumed a relatively uniform endothelial cell morphology and could be propagated and expanded. Immunocytochemical and RT-PCR analysis of differentiated cells showed that they take up acetylated low-density lipoprotein (LDL), express Flk-1, CD31 and bind the BS-I lectin. When placed in Matrigel, these Murine ES cell–derived ENDOTHELIAL CELLS formed capillary-like structures characteristic of ENDOTHELIAL CELLS.Conclusion: ES cell–derived ENDOTHELIAL CELLS provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies.

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APA: Copy

FATHI, FARDIN, POULADI, A., JAFARI KERMANI, A., & MOULA, SEYED JAVAD. (2008). DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS. CELL JOURNAL (YAKHTEH), 9(4 (36)), 262-267. SID. https://sid.ir/paper/16476/en

Vancouver: Copy

FATHI FARDIN, POULADI A., JAFARI KERMANI A., MOULA SEYED JAVAD. DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2008;9(4 (36)):262-267. Available from: https://sid.ir/paper/16476/en

IEEE: Copy

FARDIN FATHI, A. POULADI, A. JAFARI KERMANI, and SEYED JAVAD MOULA, “DIFFERENTIATION OF MURINE EMBRYONIC STEM CELLS INTO ENDOTHELIAL CELLS,” CELL JOURNAL (YAKHTEH), vol. 9, no. 4 (36), pp. 262–267, 2008, [Online]. Available: https://sid.ir/paper/16476/en

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