PREIMPLANTATION MOUSE EMBRYO CRYOPRESERVATION WITH PROPANDIOL AND SUCROSE: THE EFFECT OF DEVELOPMENTAL STAGE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NEMAT ELAHI MAHANI S.N.A.D.; REZAZADEH M.; EMAMI MEYBODI M.A.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:1999 | Volume:1 | Issue:2 Page(s): 15-20

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,909

Download:

Cites:

Information Journal Paper

Title

PREIMPLANTATION MOUSE EMBRYO CRYOPRESERVATION WITH PROPANDIOL AND SUCROSE: THE EFFECT OF DEVELOPMENTAL STAGE

Author(s)

NEMAT ELAHI MAHANI S.N.A.D. | REZAZADEH M. | EMAMI MEYBODI M.A. | Issue Writer Certificate

Keywords

EMBRYO CRYOPRESERVATIONQ4

DEVELOPMENT STAGEQ4

MOUSE EMBRYOSQ4

PROPANDIOL.Q4

Abstract

Introduction: MOUSE EMBRYOS can successfully be frozen with sucrose and PROPANDIOL. However, different developmental stages of mammalian embryos have shown to have various capacities to undergo freeze-thawing procedure. In the present study, the ability of 1,2,4 and 8-ell mouse embryo to tolerate the freeze-thawing procedure was investigated and the effect of this procedure on the further in vitro development. Materials and Methods: One, 2,4 and 8-cell MOUSE EMBRYOS were flushed from the excised uteri of the gonadotropin treated mice. Morphologically normal embryos were frozen slowly with PROPANDIOL.and sucrose and thawed rapidly. The survived embryos were cultured for 4-5 days and their development in the later stages such as hatching blastocyst was compared with non-frozen embryos. Results: The rate of survival for 1-cell embryos was higher than the other stages. The survival rates were 91, 80, 82 and 85% for 1, 2, 4 and 8-cell embryos respectively.The rate of development of the embryos beyond the late blastocyst stage was greater in 4 and 8-cell embryos. In addition to that, the 4-cell embryos developed to hatching blastocyst as it is compared with 2 and 8-cell stages embryo (P0<0.05). Conclusion: The different DEVELOPMENT STAGEs of MOUSE EMBRYOS can successfully be frozen. However, the survival rate and post-thawing development is correlated with the developmental stage of the frozen embryos.

Cites

No record.

References

Cite

APA: Copy

NEMAT ELAHI MAHANI, S.N.A.D., REZAZADEH, M., & EMAMI MEYBODI, M.A.. (1999). PREIMPLANTATION MOUSE EMBRYO CRYOPRESERVATION WITH PROPANDIOL AND SUCROSE: THE EFFECT OF DEVELOPMENTAL STAGE. CELL JOURNAL (YAKHTEH), 1(2), 15-20. SID. https://sid.ir/paper/16495/en

Vancouver: Copy

NEMAT ELAHI MAHANI S.N.A.D., REZAZADEH M., EMAMI MEYBODI M.A.. PREIMPLANTATION MOUSE EMBRYO CRYOPRESERVATION WITH PROPANDIOL AND SUCROSE: THE EFFECT OF DEVELOPMENTAL STAGE. CELL JOURNAL (YAKHTEH)[Internet]. 1999;1(2):15-20. Available from: https://sid.ir/paper/16495/en

IEEE: Copy

S.N.A.D. NEMAT ELAHI MAHANI, M. REZAZADEH, and M.A. EMAMI MEYBODI, “PREIMPLANTATION MOUSE EMBRYO CRYOPRESERVATION WITH PROPANDIOL AND SUCROSE: THE EFFECT OF DEVELOPMENTAL STAGE,” CELL JOURNAL (YAKHTEH), vol. 1, no. 2, pp. 15–20, 1999, [Online]. Available: https://sid.ir/paper/16495/en

Related Journal Papers