THE EFFECT OF CO-CULTURE OF EPITHELIAL CELLS, ISOLATED FROM AMPULLARY AND ISTHMIC REGIONS OF HUMAN OVIDUCT, ON 2-CELL MOUSE EMBRYOS

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BAHAAVAND H.; REZAZADEH M.; HOSSEINI A.

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Journal: Cell Journal (Yakhteh) Year:1999 | Volume:1 | Issue:2 Page(s): 9-14

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Title

THE EFFECT OF CO-CULTURE OF EPITHELIAL CELLS, ISOLATED FROM AMPULLARY AND ISTHMIC REGIONS OF HUMAN OVIDUCT, ON 2-CELL MOUSE EMBRYOS

Author(s)

BAHAAVAND H. | REZAZADEH M. | HOSSEINI A. | Issue Writer Certificate

Keywords

CO-CULTUREQ3

EMBRYO DEVELOPMENTQ4

OVIDUCTQ4

Abstract

Introduction: This study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human OVIDUCT during long-term CO-CULTURE on two-cell mouse embryos. Materials and Methods: Using a mechanical procedure, epithelial cells from ampullary (A) and isthmic (I) regions of the human OVIDUCT were isolated and cultured in Ham"s F-10 with 10% fetal calf serum (FSC). The 2-cell embryos were prepared from Albino Swiss mice, which were CO-CULTUREd in Ham"s F-10 with 10% FSC with either primary A-cell culture or primary I-cell for 5 days. The control group was Albino Swiss mouse embryos which were maintained in the same culture medium without co-culturing. Results: After 120h , the CO-CULTUREd embryos groups (A and I) had more blastocyts (4% an 41% respectively) and hatched embryos (22% both) as they were compared with the control which showed 16 and 8 percentages for blastocyts and hatched embryos respectively. The levels of significance were high (P<0.001 both). Moreover, in first 48h, the development rete was more in the embryos which were CO-CULTUREd with A-cell than that of I-cell (P<0.001 for 24h and p<0.01 for 48h.). Conclusion: The results suggest that an unknown factor which may be released the epithelial cell in the CO-CULTURE stimulating the development of the mouse embryos. Moreover, the in virto development of embryos may be more supported by human A-cell than human I-cell.

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APA: Copy

BAHAAVAND, H., REZAZADEH, M., & HOSSEINI, A.. (1999). THE EFFECT OF CO-CULTURE OF EPITHELIAL CELLS, ISOLATED FROM AMPULLARY AND ISTHMIC REGIONS OF HUMAN OVIDUCT, ON 2-CELL MOUSE EMBRYOS . CELL JOURNAL (YAKHTEH), 1(2), 9-14. SID. https://sid.ir/paper/16500/en

Vancouver: Copy

BAHAAVAND H., REZAZADEH M., HOSSEINI A.. THE EFFECT OF CO-CULTURE OF EPITHELIAL CELLS, ISOLATED FROM AMPULLARY AND ISTHMIC REGIONS OF HUMAN OVIDUCT, ON 2-CELL MOUSE EMBRYOS . CELL JOURNAL (YAKHTEH)[Internet]. 1999;1(2):9-14. Available from: https://sid.ir/paper/16500/en

IEEE: Copy

H. BAHAAVAND, M. REZAZADEH, and A. HOSSEINI, “THE EFFECT OF CO-CULTURE OF EPITHELIAL CELLS, ISOLATED FROM AMPULLARY AND ISTHMIC REGIONS OF HUMAN OVIDUCT, ON 2-CELL MOUSE EMBRYOS ,” CELL JOURNAL (YAKHTEH), vol. 1, no. 2, pp. 9–14, 1999, [Online]. Available: https://sid.ir/paper/16500/en

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