Molecular Identification of four important nepovirus from vineyards of Zanjan province

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Moradi Roghayeh; Koolivand Davoud; EINI OMID; HAJIZADEH MOHAMMAD

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Journal: Iranian Journal of Plant Protection Science Year:2018 | Volume:49 | Issue:1 Page(s): 99-110

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Title

Molecular Identification of four important nepovirus from vineyards of Zanjan province

Author(s)

Moradi Roghayeh | Koolivand Davoud | EINI OMID | HAJIZADEH MOHAMMAD | Issue Writer Certificate

Keywords

Arabis mosaic virusQ1

grapevine deformation virusQ1

grapevine fanleaf virusQ2

RT-PCRQ1

tomato ringspot virusQ1

Abstract

To determine incidence of nepoviruses in vineyards of zanjan province, 168 symptomatic and non-symptomatic samples were collected during season growing 2014-2015. Total RNA was extracted from the 57 leaves and green shoots of selected samples and then cDNAs were synthesized. Genomic segment of Arabis mosaic virus (ArMV), grapevine deformation virus (GDeV), Grapevine fan leaf virus (GFLV) and Tomato ring spot virus (ToRSV) was amplified by polymerase chain reaction (PCR) using specific primers. The result revealed that the expected bands were amplified at 15. 7, 15. 7, 10. 5 and 14% of different samples belong to ArMV, GDeV, GFLV and ToRSV, respectively. Following sequencing and multiple alignment of nucleotide sequence of amplified fragments with isolates that retrieved from NCBI. Phylogenetic tree was created by MEGA6 software using Neighbour-Joining method. The result revealed that the Iranian isolates were grouped with reported isolates from different regions around the world based on geographical. This is the first comprehensive study based on molecular methods to determine grapevine nepoviruses in Zanjan province and the first report of GDeV from Zanjna based on our knowledge.

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APA: Copy

Moradi, Roghayeh, Koolivand, Davoud, EINI, OMID, & HAJIZADEH, MOHAMMAD. (2018). Molecular Identification of four important nepovirus from vineyards of Zanjan province. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(1 ), 99-110. SID. https://sid.ir/paper/171687/en

Vancouver: Copy

Moradi Roghayeh, Koolivand Davoud, EINI OMID, HAJIZADEH MOHAMMAD. Molecular Identification of four important nepovirus from vineyards of Zanjan province. IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2018;49(1 ):99-110. Available from: https://sid.ir/paper/171687/en

IEEE: Copy

Roghayeh Moradi, Davoud Koolivand, OMID EINI, and MOHAMMAD HAJIZADEH, “Molecular Identification of four important nepovirus from vineyards of Zanjan province,” IRANIAN JOURNAL OF PLANT PROTECTION SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 1 , pp. 99–110, 2018, [Online]. Available: https://sid.ir/paper/171687/en

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