THE POSSIBLE ROLE OF PHOSPHOENOLPYROVATE CARBOXYKINASE (PEPCK) IN PROTEIN CONTENT OF CHICKPEA SEEDS (CICER ARIETINUM L.)

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BEYHAGHI MARIA; BAGHERI A.A.R.; BAHRAMI AHMAD REZA; SHAHRIARI F.A.; NEZAMI AHMAD

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Journal: IRANIAN JOURNAL OF PULSES RESEARCH Year:2010 | Volume:1 | Issue:1 Page(s): 57-64

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Title

THE POSSIBLE ROLE OF PHOSPHOENOLPYROVATE CARBOXYKINASE (PEPCK) IN PROTEIN CONTENT OF CHICKPEA SEEDS (CICER ARIETINUM L.)

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Keywords

CHICKPEAQ3

NITROGEN METABOLISMQ3

PHOSPHOENOLPYRUVATE CARBOXYKINASEQ3

SEED DEVELOPMENTQ3

Abstract

Phosphoenolpyruvate Carboxykinase (PEPCK) has been shown to be present in plants and animals, playing different metabolic roles in different tissues. The basic role of this enzyme is its contribution in the gluconeogenesis pathway. Evidences have shown that PEPCK may play a role in metabolism of nitrogenous compounds in developing seeds of legumes. In this research, pepck gene expression and the occurrence of PEPCK protein and its activity in different genotypes of CHICKPEA (Cicer arietinume L.) were determined. Two low protein genotypes (MCC291 & MCC373) and two high protein genotypes (MCC458 & MCC053) out of 20 CHICKPEA genotypes were selected, from which the total RNA was extracted through different stages of SEED DEVELOPMENT. The expression of CHICKPEA pepck gene was estimated by semi-quantitative RT-PCR. The results of RT-PCR showed that two isoforms of this gene were expressed in high protein genotypes, whereas in the low protein genotypes were not expressed. The differential expression of pepck gene is perhaps related to the possible role of Phosphoenolpyrovate Carboxykinase in protein content of CHICKPEA seeds.

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APA: Copy

BEYHAGHI, MARIA, BAGHERI, A.A.R., BAHRAMI, AHMAD REZA, SHAHRIARI, F.A., & NEZAMI, AHMAD . (2010). THE POSSIBLE ROLE OF PHOSPHOENOLPYROVATE CARBOXYKINASE (PEPCK) IN PROTEIN CONTENT OF CHICKPEA SEEDS (CICER ARIETINUM L.). IRANIAN JOURNAL OF PULSES RESEARCH, 1(1), 57-64. SID. https://sid.ir/paper/171795/en

Vancouver: Copy

BEYHAGHI MARIA, BAGHERI A.A.R., BAHRAMI AHMAD REZA, SHAHRIARI F.A., NEZAMI AHMAD . THE POSSIBLE ROLE OF PHOSPHOENOLPYROVATE CARBOXYKINASE (PEPCK) IN PROTEIN CONTENT OF CHICKPEA SEEDS (CICER ARIETINUM L.). IRANIAN JOURNAL OF PULSES RESEARCH[Internet]. 2010;1(1):57-64. Available from: https://sid.ir/paper/171795/en

IEEE: Copy

MARIA BEYHAGHI, A.A.R. BAGHERI, AHMAD REZA BAHRAMI, F.A. SHAHRIARI, and AHMAD NEZAMI, “THE POSSIBLE ROLE OF PHOSPHOENOLPYROVATE CARBOXYKINASE (PEPCK) IN PROTEIN CONTENT OF CHICKPEA SEEDS (CICER ARIETINUM L.),” IRANIAN JOURNAL OF PULSES RESEARCH, vol. 1, no. 1, pp. 57–64, 2010, [Online]. Available: https://sid.ir/paper/171795/en

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