ASSESSING THE LEVELS OF CREB AFTER SIRNA MEDIATED KNOCKDOWN IN K562 CELLS

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DEYLAMI Z.; BANAN M.; ASGHARIYAN A.; GHARAH SORAN J.; JAVADI GH.; KAHRIZI K.; NAJM ABADI H.

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Journal: JOURNAL OF ANIMAL BIOLOGY Year:2008 | Volume:1 | Issue:1 Page(s): 29-34

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Title

ASSESSING THE LEVELS OF CREB AFTER SIRNA MEDIATED KNOCKDOWN IN K562 CELLS

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Keywords

PROTEIN CREBQ3

SIRNAQ2

HELA CELLSQ3

Abstract

Background: CREB is an important downstream protein for many signaling pathways. By having efficient SIRNAs against CREB, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. In this research the efficiency of CREB knockdown by 2 different SIRNAs in HELA CELLS has been studied.Materials and methods: HELA CELLS were transfected by SIRNAs using Lipofectamine 2000. RNA extraction and cDNA synthesis have been done. The efficiency of CREB knockdown has been assessed by quantitative relative real time PCR. According to our results only one of the SIRNAs has a high level of inhibitory effect on CREB gene expression in HELA CELLS. The expression of CREB by this SIRNA was knocked-down by 64.4% in HELA CELLS.Conclusion: By using efficient SIRNA specifically for knocking down of CREB gene, we can apply this efficient SIRNA in different cell types due to study of signaling pathway which CREB may be involved.

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APA: Copy

DEYLAMI, Z., BANAN, M., ASGHARIYAN, A., GHARAH SORAN, J., JAVADI, GH., KAHRIZI, K., & NAJM ABADI, H.. (2008). ASSESSING THE LEVELS OF CREB AFTER SIRNA MEDIATED KNOCKDOWN IN K562 CELLS. JOURNAL OF ANIMAL BIOLOGY, 1(1), 29-34. SID. https://sid.ir/paper/176681/en

Vancouver: Copy

DEYLAMI Z., BANAN M., ASGHARIYAN A., GHARAH SORAN J., JAVADI GH., KAHRIZI K., NAJM ABADI H.. ASSESSING THE LEVELS OF CREB AFTER SIRNA MEDIATED KNOCKDOWN IN K562 CELLS. JOURNAL OF ANIMAL BIOLOGY[Internet]. 2008;1(1):29-34. Available from: https://sid.ir/paper/176681/en

IEEE: Copy

Z. DEYLAMI, M. BANAN, A. ASGHARIYAN, J. GHARAH SORAN, GH. JAVADI, K. KAHRIZI, and H. NAJM ABADI, “ASSESSING THE LEVELS OF CREB AFTER SIRNA MEDIATED KNOCKDOWN IN K562 CELLS,” JOURNAL OF ANIMAL BIOLOGY, vol. 1, no. 1, pp. 29–34, 2008, [Online]. Available: https://sid.ir/paper/176681/en

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