DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SAMPLES USING REAL TIME PCR TECHNIQUES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOUSAVI M.; AMROLLAHI H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ANIMAL BIOLOGY Year:2016 | Volume:8 | Issue:4 Page(s): 95-103

Download Full-Text

Persian Verion

View:

618

Download:

Cites:

Information Journal Paper

Title

DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SAMPLES USING REAL TIME PCR TECHNIQUES

Author(s)

MOUSAVI M. | AMROLLAHI H. | Issue Writer Certificate

Keywords

RPOBQ1

KATGQ1

IS6110Q1

REAL TIME PCRQ1

MDR-TBQ1

CTAB TECHNIQUEQ1

Abstract

Tuberculosis is an infectious disease is created by Mycobacterium tuberculosis. It can be resistant to antimicrobials drugs that used to treat the disease. Multidrug resistant tuberculosis (MDR-TB) means resistant to the drugs isoniazid and rifampin. The purpose of this study was to investigate the presence or absence of any contaminant patients through IS6110 fragment in the sample and also the presence or absence of resistance to the antibiotics rifampin and isoniazid in the sample with REAL TIME PCR technique. Sputum samples were collected from patients with tuberculosis were cultured in medium L-J And extracted genomic DNA using CTAB TECHNIQUE was performed to determine the resistance or non-resistance samples were analyzed by REAL TIME PCR. Samples using conventional PCR technique using specific primers for the detection of IS6110 examined whether being tubersulosis or non-tuberculosis. Study of 21 Clinical cases, demonstrate that 17 cases was Mycobacterium Tuberculosis and 4 were non Tuberculosis. All samples were also sensitive to the antibiotic. Using REAL TIME PCR diagnostic techniques are useful for the study of antibiotic resistance genes in the shortest possible time (less than a day) and high specificity and accuracy at the time of diagnosis can be done. Fortunately in this study all samples were sensitive to all antibiotics rifampin and isoniazid the probable cause of this result can because of successfully controlled disease by medical centers through successive examinations of patients and the low statistics of immigrants in the province.

Cites

No record.

References

No record.

Cite

APA: Copy

MOUSAVI, M., & AMROLLAHI, H.. (2016). DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SAMPLES USING REAL TIME PCR TECHNIQUES. JOURNAL OF ANIMAL BIOLOGY, 8(4), 95-103. SID. https://sid.ir/paper/176771/en

Vancouver: Copy

MOUSAVI M., AMROLLAHI H.. DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SAMPLES USING REAL TIME PCR TECHNIQUES. JOURNAL OF ANIMAL BIOLOGY[Internet]. 2016;8(4):95-103. Available from: https://sid.ir/paper/176771/en

IEEE: Copy

M. MOUSAVI, and H. AMROLLAHI, “DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SAMPLES USING REAL TIME PCR TECHNIQUES,” JOURNAL OF ANIMAL BIOLOGY, vol. 8, no. 4, pp. 95–103, 2016, [Online]. Available: https://sid.ir/paper/176771/en

Related Journal Papers