THE FREQUENCY OF TEM-L GENE IN EXTENDED SPECTRUM BETA LACTAMASES PRODUCING ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE AND ENTEROBACTER STRAINS ISOLATED FROM HOSPITAL CLINICAL SAMPLES USING PCR

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ZAMANZAD B.; DEYHAM B.; NAFISI M.R.; KARIMI ALI; FAROKHI EFAT

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Journal: Avicenna Journal of Clinical Medicine Year:2008 | Volume:14 | Issue:4 (SN 46) Page(s): 19-25

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Title

THE FREQUENCY OF TEM-L GENE IN EXTENDED SPECTRUM BETA LACTAMASES PRODUCING ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE AND ENTEROBACTER STRAINS ISOLATED FROM HOSPITAL CLINICAL SAMPLES USING PCR

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Keywords

ENTEROBACTERIACEASEQ3

EXTENDED SPECTRUM BETA LACTAMASESQ3

TEM-1 BETA LACTAMASE GENEQ3

Abstract

Introduction & Objective: TEM-1 BETA LACTAMASE GENE is one of the important plasmidic genes in Enterobacteriaceae which is the cause of over 90% of Escherichia coli isolates resistance to beta lactam antibiotics. In this study, the frequency of TEM-1 gene in nosocomial strains of Escherichia coli, Klebsiella pneumoniae and Enterobacter was investigated using PCR.Materials & Methods: In this descriptive -analytical study, 83 ESBLs producing enteric bacteria (Escherichia coli, Klebsiella pneumoniae and Enterobacter strains) isolated from hospital clinical samples were studied for the presence of TEM-1 gene using TEM-1 beta lactamase specific primers (Gene-Fanavaran, Iran) using PCR. The detection of 1079 bp bands in poly acrylamide gel electrophoresis was considered 'as presence of TEM-1 gepe in bacterial isolates. Data were analyzed using Chi-Square test. Results: TEM-1 gene was detected in 54.2% (45) of isolated bacteria. The frequency of TEM- 1 gene in different isolates was not significant. TEM-1 gene was detected in 62.5 % Of Enterobacter, 58.3% of K.pneumoniae and 48.7% of E.coli isolates.Conclusion: This study showed that, TEM-1 gene encodes over 50% of ESBLs in. enteric bacteria. Therefore, we recommend detection of this gene as a routine bacteriologic procedure in management of the nosocomial infections caused by enteric bacteria.

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APA: Copy

ZAMANZAD, B., DEYHAM, B., NAFISI, M.R., KARIMI, ALI, & FAROKHI, EFAT. (2008). THE FREQUENCY OF TEM-L GENE IN EXTENDED SPECTRUM BETA LACTAMASES PRODUCING ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE AND ENTEROBACTER STRAINS ISOLATED FROM HOSPITAL CLINICAL SAMPLES USING PCR. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 14(4 (SN 46)), 19-25. SID. https://sid.ir/paper/17795/en

Vancouver: Copy

ZAMANZAD B., DEYHAM B., NAFISI M.R., KARIMI ALI, FAROKHI EFAT. THE FREQUENCY OF TEM-L GENE IN EXTENDED SPECTRUM BETA LACTAMASES PRODUCING ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE AND ENTEROBACTER STRAINS ISOLATED FROM HOSPITAL CLINICAL SAMPLES USING PCR. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2008;14(4 (SN 46)):19-25. Available from: https://sid.ir/paper/17795/en

IEEE: Copy

B. ZAMANZAD, B. DEYHAM, M.R. NAFISI, ALI KARIMI, and EFAT FAROKHI, “THE FREQUENCY OF TEM-L GENE IN EXTENDED SPECTRUM BETA LACTAMASES PRODUCING ESCHERICHIA COLI, KLEBSIELLA PNEUMONIAE AND ENTEROBACTER STRAINS ISOLATED FROM HOSPITAL CLINICAL SAMPLES USING PCR,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 14, no. 4 (SN 46), pp. 19–25, 2008, [Online]. Available: https://sid.ir/paper/17795/en

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