ISOLATION AND EXPRESSION ANALYSIS OF ALSOS1 GENE UNDER NACL STRESS IN HALOPHYTE GRASS AELUROPUS LITTORALIS PARL.

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GHASEMI OMRAN VALIOLLAH; BAGHERI ABDOLREZA; NEMATZADEH GHORBAN ALI; MIRSHAMSI AMIN; BABAEIAN JELODAR NADALI

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Journal: JOURNAL OF CROP BREEDING Year:2016 | Volume:7 | Issue:16 Page(s): 60-69

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Title

ISOLATION AND EXPRESSION ANALYSIS OF ALSOS1 GENE UNDER NACL STRESS IN HALOPHYTE GRASS AELUROPUS LITTORALIS PARL.

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Keywords

SALT STRESSQ2

AELUROPUS LITTORALISQ1

ALSOS1Q2

EXPRESSIONQ1

Abstract

We have isolated a Na+/H+antiporter gene from AELUROPUS LITTORALIS under NaCl SALT STRESS.The isolated cDNA is 3981 bp long and contains a 3438 bp open reading frame. The amino acid sequences of PtNHA1 show high identities to SOS1 plant transporters. PtNHA1 is predicted to contain 12 hypothetical transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail in the C-terminal part. The EXPRESSION pattern of this gene was investigated upon 250 mM NaCl treatment after 6 h and 1, 3, 8 and 17 days time courses. EXPRESSION of ALSOS1 greatly increased in leaves after 6 h. In node and internode tissues the transcript levels of ALSOS1 peaked at 24 h after salt treatment and then gradually decreased within 3 and 8 days and finally after 17 days reached to a steady-state in which the mRNA content was similar to that of control plants. The transcript abundance of this gene in roots slightly increased upon salt treatment and after 3 days reached to its maximum levels and this EXPRESSION continued until 8 days and then decreased to reach a new steady-state in which the mRNA content was about 2- fold that of control plants.

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APA: Copy

GHASEMI OMRAN, VALIOLLAH, BAGHERI, ABDOLREZA, NEMATZADEH, GHORBAN ALI, MIRSHAMSI, AMIN, & BABAEIAN JELODAR, NADALI. (2016). ISOLATION AND EXPRESSION ANALYSIS OF ALSOS1 GENE UNDER NACL STRESS IN HALOPHYTE GRASS AELUROPUS LITTORALIS PARL.. JOURNAL OF CROP BREEDING, 7(16), 60-69. SID. https://sid.ir/paper/181049/en

Vancouver: Copy

GHASEMI OMRAN VALIOLLAH, BAGHERI ABDOLREZA, NEMATZADEH GHORBAN ALI, MIRSHAMSI AMIN, BABAEIAN JELODAR NADALI. ISOLATION AND EXPRESSION ANALYSIS OF ALSOS1 GENE UNDER NACL STRESS IN HALOPHYTE GRASS AELUROPUS LITTORALIS PARL.. JOURNAL OF CROP BREEDING[Internet]. 2016;7(16):60-69. Available from: https://sid.ir/paper/181049/en

IEEE: Copy

VALIOLLAH GHASEMI OMRAN, ABDOLREZA BAGHERI, GHORBAN ALI NEMATZADEH, AMIN MIRSHAMSI, and NADALI BABAEIAN JELODAR, “ISOLATION AND EXPRESSION ANALYSIS OF ALSOS1 GENE UNDER NACL STRESS IN HALOPHYTE GRASS AELUROPUS LITTORALIS PARL.,” JOURNAL OF CROP BREEDING, vol. 7, no. 16, pp. 60–69, 2016, [Online]. Available: https://sid.ir/paper/181049/en

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