Identification, Isolation and Bioinformatic Analysis of Specific Tuber Promoter in Plants

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Ghanbari Moheb Seraj Rahele; TOHIDFAR MASOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: JOURNAL OF CROP BREEDING Year:2020 | Volume:11 | Issue:32 Page(s): 1-10

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Title

Identification, Isolation and Bioinformatic Analysis of Specific Tuber Promoter in Plants

Author(s)

Ghanbari Moheb Seraj Rahele | TOHIDFAR MASOUD | Issue Writer Certificate

Keywords

Cis ElementsQ1

PatatinQ1

Sweet PotatoQ1

TaroQ1

Transcription FactorQ1

Abstract

In this study, in order to find the suitable tuber promoter, an experiment was conducted in Shahid Beheshti University in. For this purpose, promoter sequences of different tuberous plants were searched at NCBI. Sequences were multiple-aligned and the target primers designed from conserved regions. PCR analysis confirmed the presence of the desired promoter in plants of Sweet Potato and Taro. In order to further confirmation, bioinformatic analysis including identification of Cis Elements, Transcription Factors, their function, Transcription Factors with high and low expression were performed. Finally, the identified promoter was compared with Patatin class promoter in terms of Cis Elements and function. Results of bioinformatic analysis indicated that the promoter has specific and general Cis Elements. General Cis Elements that are present in most promoters and are involved in activation and regulation of promoters, cellular processes, developmental processes and tolerance to biological and non-biological stresses. Special Cis Elements of tuber are active in expression of storage protein and starch. Comparison between studied promoter and specific tuber promoter of Patatin confirms activity of the studied promoter in the tuber. A tuber promoter can be used to express various proteins in tuber plants in the future.

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APA: Copy

Ghanbari Moheb Seraj, Rahele, & TOHIDFAR, MASOUD. (2020). Identification, Isolation and Bioinformatic Analysis of Specific Tuber Promoter in Plants. JOURNAL OF CROP BREEDING, 11(32 ), 1-10. SID. https://sid.ir/paper/181234/en

Vancouver: Copy

Ghanbari Moheb Seraj Rahele, TOHIDFAR MASOUD. Identification, Isolation and Bioinformatic Analysis of Specific Tuber Promoter in Plants. JOURNAL OF CROP BREEDING[Internet]. 2020;11(32 ):1-10. Available from: https://sid.ir/paper/181234/en

IEEE: Copy

Rahele Ghanbari Moheb Seraj, and MASOUD TOHIDFAR, “Identification, Isolation and Bioinformatic Analysis of Specific Tuber Promoter in Plants,” JOURNAL OF CROP BREEDING, vol. 11, no. 32 , pp. 1–10, 2020, [Online]. Available: https://sid.ir/paper/181234/en

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