PRODUCTION AND SERUM EVALUATION OF RECOMBINANT 18KDA OUTER MEMBRANE LIPOPROTEIN (OMP19) OF BRUCELLA ABORTUS S19 IN BALB/C MICE MODEL

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FARAHI F.; ASLI E.; MOHABATI MOBAREZ A.; BAKHTIARI A.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:3 | Issue:8 Page(s): 23-28

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Title

PRODUCTION AND SERUM EVALUATION OF RECOMBINANT 18KDA OUTER MEMBRANE LIPOPROTEIN (OMP19) OF BRUCELLA ABORTUS S19 IN BALB/C MICE MODEL

Author(s)

FARAHI F. | ASLI E. | MOHABATI MOBAREZ A. | BAKHTIARI A. | Issue Writer Certificate

Keywords

BRUCELLOSISQ2

BRUCELLA ABORTUSQ3

ROMP19Q2

Abstract

Background and Objective: BRUCELLOSIS is a zoonotic disease which has an extremely economic importance. Prevention and diagnosis of human and cattle infected with the organism both are challenging researches. Immunologic evaluation of diverse antigens of Brucella cells has a key role in development of prevention and diagnosis programs. Here, we report the production and immunologic evaluation of recombinant 18kDa outer membrane lipoprotein ofBrucella abortus (Omp19).Materials & Methods: Brucella omp19 gene was amplified with PrimSTAR ® HS DNA polymerase, cloned in pJET 1.2 and sequenced. The omp19 gene was cut by BamHI and HindIII from confirmed vectors and subcloned in pET28a (+). pET28a (+) vectors cloned with omp19 were transformed into E. coli BL21 (DE3) as expression host. Expression of recombinant protein was induced by IPTG and verified by western-blotting. Recombinant protein was purified using Ni-NTA resin and was applied to detect antibodies in human and animal BRUCELLOSIS cases serum samples via western-blotting.Results: Recognition of antibody to the recombinant Omp19 (rOmp19) in serum samples of infected cases suggests the stimulation of immune response to this protein during BRUCELLOSIS.Conclusion: The study indicates ROMP19 of BRUCELLA ABORTUS is an appropriate antigen for further investigations in vaccines and serologic diagnosis programs.

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APA: Copy

FARAHI, F., ASLI, E., MOHABATI MOBAREZ, A., & BAKHTIARI, A.. (2011). PRODUCTION AND SERUM EVALUATION OF RECOMBINANT 18KDA OUTER MEMBRANE LIPOPROTEIN (OMP19) OF BRUCELLA ABORTUS S19 IN BALB/C MICE MODEL. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(8), 23-28. SID. https://sid.ir/paper/181440/en

Vancouver: Copy

FARAHI F., ASLI E., MOHABATI MOBAREZ A., BAKHTIARI A.. PRODUCTION AND SERUM EVALUATION OF RECOMBINANT 18KDA OUTER MEMBRANE LIPOPROTEIN (OMP19) OF BRUCELLA ABORTUS S19 IN BALB/C MICE MODEL. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(8):23-28. Available from: https://sid.ir/paper/181440/en

IEEE: Copy

F. FARAHI, E. ASLI, A. MOHABATI MOBAREZ, and A. BAKHTIARI, “PRODUCTION AND SERUM EVALUATION OF RECOMBINANT 18KDA OUTER MEMBRANE LIPOPROTEIN (OMP19) OF BRUCELLA ABORTUS S19 IN BALB/C MICE MODEL,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 8, pp. 23–28, 2011, [Online]. Available: https://sid.ir/paper/181440/en

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