INVESTIGATING THE FUNCTION OF CONVENTIONAL ANTIBIOTICS ACCORDING TO NCCLS STANDARDS IN EVALUATION OF SENSITIVITY OF STANDARD & CLINICAL KLEBSIELLA PNEUMONIA, PSEUDOMONAS AERUGINOSA MICROBIAL SUSHES

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FOROOHI F.; ZAMANI H.; JAMALIFAR H.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 53-58

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Title

INVESTIGATING THE FUNCTION OF CONVENTIONAL ANTIBIOTICS ACCORDING TO NCCLS STANDARDS IN EVALUATION OF SENSITIVITY OF STANDARD & CLINICAL KLEBSIELLA PNEUMONIA, PSEUDOMONAS AERUGINOSA MICROBIAL SUSHES

Author(s)

FOROOHI F. | ZAMANI H. | JAMALIFAR H. | Issue Writer Certificate

Keywords

ANTIBIOTICQ2

PSEUDOMONAS AERUGINOSAQ2

KLEBSIELLA PNEUMONIAQ2

NCCLSQ2

Abstract

Background and Objective: Although using ANTIBIOTICs made a superior progress in medicinal industry, but their massive and false prescription results bacterial resistance to consumption ANTIBIOTICs. So using one method for specifying sensitivity is not trusty alone. The aim of this study is evaluation of the resistant of the clinical strains PSEUDOMONAS AERUGINOSA and KLEBSIELLA PNEUMONIA to common ANTIBIOTICs and determining the common ANTIBIOTICs and degree of increase MIC such as imipeneme and ciprofloxacin against these bacteria.Materials and Methods: In this study, at first 50 samples of PSEUDOMONAS AERUGINOSA and 50 samples of KLEBSIELLA PNEUMONIA from different clinical specimens such as wounds, ear discharge, eye discharge, about patients in the ICU and CCU at hospitals were taken from each sample to identify the exact genus and species of bacteria, was in fully aseptic conditions on this Mac Kancky agar culture medium for growth of KLEBSIELLA PNEUMONIA and Strimid agar for Pseudomonas with four regional basis. After mass culturing purified colonies, separating and specifying Pseudomonas strain with biochemical methods. Then, in order to specify the anti microbial sensitivity of the considered ANTIBIOTICs, created no growth halo on the plate were measured in millimeter.Results: The Analysis on bacterial resistance of PSEUDOMONAS AERUGINOSA in Rifampin with rate (0.5 mg/L) showed that there is decrease on halo of growth around the ANTIBIOTIC Rifampin disks also has been reported significant in overall E. coli strains at the lower level and on a higher level of resistance in KLEBSIELLA PNEUMONIA, while about the Tetracycline and Chloramphenicol and Ceftazidime ANTIBIOTICs has not been reported these resistance.Conclusion: It was considered that PSEUDOMONAS AERUGINOSA strains showed resistance to Rifampin in thicker halos and KLEBSIELLA PNEUMONIA strains showed resistance to Tetracycline in thinner ones, as well.

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APA: Copy

FOROOHI, F., ZAMANI, H., & JAMALIFAR, H.. (2012). INVESTIGATING THE FUNCTION OF CONVENTIONAL ANTIBIOTICS ACCORDING TO NCCLS STANDARDS IN EVALUATION OF SENSITIVITY OF STANDARD & CLINICAL KLEBSIELLA PNEUMONIA, PSEUDOMONAS AERUGINOSA MICROBIAL SUSHES. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 53-58. SID. https://sid.ir/paper/181462/en

Vancouver: Copy

FOROOHI F., ZAMANI H., JAMALIFAR H.. INVESTIGATING THE FUNCTION OF CONVENTIONAL ANTIBIOTICS ACCORDING TO NCCLS STANDARDS IN EVALUATION OF SENSITIVITY OF STANDARD & CLINICAL KLEBSIELLA PNEUMONIA, PSEUDOMONAS AERUGINOSA MICROBIAL SUSHES. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):53-58. Available from: https://sid.ir/paper/181462/en

IEEE: Copy

F. FOROOHI, H. ZAMANI, and H. JAMALIFAR, “INVESTIGATING THE FUNCTION OF CONVENTIONAL ANTIBIOTICS ACCORDING TO NCCLS STANDARDS IN EVALUATION OF SENSITIVITY OF STANDARD & CLINICAL KLEBSIELLA PNEUMONIA, PSEUDOMONAS AERUGINOSA MICROBIAL SUSHES,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 53–58, 2012, [Online]. Available: https://sid.ir/paper/181462/en

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