PRODUCTION AND PURIFICATION OF TAX PROTEIN OF HTLV-1 RETROVIRUS USING RECOMBINANT DNA TECHNOLOGY AND IMMUNOAFFINITY CHROMATOGRAPHY

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ZOLFAGHARY M.R.; KHOSHROO M.; VARASTEH A.R.; ABBASZADEGAN M.R.; SAFARI M.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2012 | Volume:4 | Issue:13 Page(s): 1-6

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Title

PRODUCTION AND PURIFICATION OF TAX PROTEIN OF HTLV-1 RETROVIRUS USING RECOMBINANT DNA TECHNOLOGY AND IMMUNOAFFINITY CHROMATOGRAPHY

Author(s)

Keywords

HTLV-1Q2

TAX PROTEINQ2

RECOMBINANT DNAQ2

IMMUNOAFFINITY CHROMATOGRAPHYQ2

Abstract

Background and Objective: Infection of T cells by human T cell lymphotropic virus type1 (HTLV-1) immortalizes these cells as a prelude to adult T cell leukemia (ATL). Transformation of T cells in the early phases is mediated by the HTLV-1 TAX PROTEIN. Khorasan province, and particularly Mashhad is an endemic area for HTLV-1 with an incidence of approximately 5%. TAX PROTEIN is useful to produce diagnostic kits for screening of infections, and it can also be applied for to following up anti-Tax antibodies in patients. Therefore the purpose of present research was to produce of TAX PROTEIN which is a good immunogen.Materials and Methods: After transformation of E.Coli with plasmid containing Tax gene, PCR reaction was done to determine whether transformation was occurred. Then, transformed bacteria were grown on LB medium and the expressed protein was precipitated with ammonium sulfate. IMMUNOAFFINITY CHROMATOGRAPHY was used to protein purification, and silver staining and dot blotting were used for the characterization of TAX PROTEIN.Results: Results of PCR reaction done with tax gene specific primers showed bacterial transformation, and also the results of silver staining and dot blotting showed the purified TAX PROTEIN which was done by affinity chromatography.Conclusion: The results show that although TAX PROTEIN was produced by this method, the amounts of the produced protein were low.

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APA: Copy

ZOLFAGHARY, M.R., KHOSHROO, M., VARASTEH, A.R., ABBASZADEGAN, M.R., & SAFARI, M.. (2012). PRODUCTION AND PURIFICATION OF TAX PROTEIN OF HTLV-1 RETROVIRUS USING RECOMBINANT DNA TECHNOLOGY AND IMMUNOAFFINITY CHROMATOGRAPHY. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 4(13), 1-6. SID. https://sid.ir/paper/181463/en

Vancouver: Copy

ZOLFAGHARY M.R., KHOSHROO M., VARASTEH A.R., ABBASZADEGAN M.R., SAFARI M.. PRODUCTION AND PURIFICATION OF TAX PROTEIN OF HTLV-1 RETROVIRUS USING RECOMBINANT DNA TECHNOLOGY AND IMMUNOAFFINITY CHROMATOGRAPHY. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2012;4(13):1-6. Available from: https://sid.ir/paper/181463/en

IEEE: Copy

M.R. ZOLFAGHARY, M. KHOSHROO, A.R. VARASTEH, M.R. ABBASZADEGAN, and M. SAFARI, “PRODUCTION AND PURIFICATION OF TAX PROTEIN OF HTLV-1 RETROVIRUS USING RECOMBINANT DNA TECHNOLOGY AND IMMUNOAFFINITY CHROMATOGRAPHY,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 4, no. 13, pp. 1–6, 2012, [Online]. Available: https://sid.ir/paper/181463/en

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