Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests

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NARIMANI B.; HOGHOOGHI RAD N.; SHAYAN P.; RAHBARI S.; ESMAEIL NIA K.

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Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2019 | Volume:13 | Issue:1 (49) Page(s): 41-55

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Title

Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests

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Keywords

Theileria orientalisQ2

BuffaloQ3

Microscopic examinationQ1

Molecular detectionQ1

West AzarbaijanQ2

Abstract

The causative agent of bovine theileriosis is obligatory intracellular protozoa called T. annulata which results in severe anemia, severe pulmonary edema and rapid death in non-native animals. The present study was aimed to detect the current state of Theileria infection in indigenous Buffaloes and its association with environmental factors in four cities of West Azarbaijan province using microscopic tests (blood smear evaluation) and molecular assays. Blood samples were randomly collected from 291 Buffaloes in the region, during March to July 2014. Direct and semi-nested PCR techniques were used to detect DNA of Theileria spp. using specific pair primer 18SrRNA. In the microscopic assay method, presence of this parasite in four (1. 37%) Buffaloes was approved. The molecular assays revealed that 4 out of 291 animals (1. 37%) were infected by T. annulata. Also the present study showed that two female adult Buffaloes (0. 68%) were simultaneously infected by T. annulata and T. orientalis. Apparent and real prevalence of infection of Buffaloes to T. annulata in the molecular method were 1. 37 and 1 percent respectively. The chance of infection by T. annulata and T. orientalis were equal. Molecular prevalence of T. annulata in Urmia and Salmas were 2. 2 and 2. 3 percent respectively (p<0. 05). Molecular prevalence of T. orientalis in Urmia was 2. 2%. This is the first study to indicate the presence of infection with T. orientalis in indigenous Buffaloes of Urmia city in Iran.

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APA: Copy

NARIMANI, B., HOGHOOGHI RAD, N., SHAYAN, P., RAHBARI, S., & ESMAEIL NIA, K.. (2019). Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 13(1 (49) ), 41-55. SID. https://sid.ir/paper/183023/en

Vancouver: Copy

NARIMANI B., HOGHOOGHI RAD N., SHAYAN P., RAHBARI S., ESMAEIL NIA K.. Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2019;13(1 (49) ):41-55. Available from: https://sid.ir/paper/183023/en

IEEE: Copy

B. NARIMANI, N. HOGHOOGHI RAD, P. SHAYAN, S. RAHBARI, and K. ESMAEIL NIA, “Detection of Theileria orientalis infection in indigenous buffaloes of West Azarbaijan province using molecular and microscopic tests,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 13, no. 1 (49) , pp. 41–55, 2019, [Online]. Available: https://sid.ir/paper/183023/en

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