The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line (CF41. Mg)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mostahsan z.; MORTAZAVI P.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2019 | Volume:13 | Issue:1 (49) Page(s): 1-14

Download Full-Text

Persian Verion

View:

510

Download:

Cites:

Information Journal Paper

Title

The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line (CF41. Mg)

Author(s)

Mostahsan z. | MORTAZAVI P. | Issue Writer Certificate

Keywords

Tilia extractQ1

Caspase 3Q1

Caspase 9Q1

Canine mammary gland cancer cell lineQ1

Abstract

Mammary gland tumors arethe most common type of cancer in dogs and cats after skin tumors. Any disorder in the process of apoptosis causes the growth of cancer cells. Caspases 3 and 9 play an important role in the process of apoptosis as initiator and executioner caspases. This study was conducted to investigate the effect of Tilia (Tilia plathyphyllos) extract on gene expression of Caspase 3 and Caspase 9 in Canine mammary gland cancer cell line. Canine mammary gland tumor cells (CF41. Mg) were passaged in the presence of 5, 10, 25, 50 and 100 mg/ml of Tilia extract for 24, 48 and 72 hours. Microculture tetrajolium test (MTT) was used after the passage to assay the number of live cells. To understand the mechanisms involved in induction of cell death at previously mentioned intervals, RNA was extracted and cDNA synthetizedand the rate of gene expression of Caspase 3 and 9 was evaluated using specific primers with RT-PCR. According to the result, maximum anti-tumor activity of this extract was observed after 48 hours with the dose of 25 mg/ml. RT-PCR results indicated gene expression of Caspase 3 and 9 after 24, 48 and 72 hours in the treated samples. The highest extent of gene expression was observed at 10 and 100 mg/ml of extract for Caspase 3 and 10 mg/ml of extract for Caspase 9. The results of the present study confirmed the inhibitory effects of Tilia extract on growth of canine mammary gland tumor cell line.

Cites

No record.

References

No record.

Cite

APA: Copy

Mostahsan, z., & MORTAZAVI, P.. (2019). The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line (CF41. Mg). VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 13(1 (49) ), 1-14. SID. https://sid.ir/paper/183025/en

Vancouver: Copy

Mostahsan z., MORTAZAVI P.. The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line (CF41. Mg). VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2019;13(1 (49) ):1-14. Available from: https://sid.ir/paper/183025/en

IEEE: Copy

z. Mostahsan, and P. MORTAZAVI, “The effects of Tilia (Tilia plathyphyllos) extract on gene expression of caspase 3 and caspase 9 in canine mammary gland cancer cell line (CF41. Mg),” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 13, no. 1 (49) , pp. 1–14, 2019, [Online]. Available: https://sid.ir/paper/183025/en

Related Journal Papers