Effect of methionine deficiency on small intestinal histology in Japanese quail

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Khalkhali Ashkan; hamedi somayeh; paryani mohammadreza

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Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2020 | Volume:13 | Issue:4 (52) Page(s): 341-352

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Title

Effect of methionine deficiency on small intestinal histology in Japanese quail

Author(s)

Khalkhali Ashkan | hamedi somayeh | paryani mohammadreza | Issue Writer Certificate

Keywords

Methionine deficiencyQ1

Small intestineQ2

HistologyQ2

Japanese quailQ1

Abstract

Materials and methods: To evaluate the Met deficiency as a vital amino acid on Histology of Small intestine of Japanese quail, 20 male one-day old quails were randomly allocated into 2 groups of 10 birds. One group received Met deficient diet while another group of birds were kept as control with standard diet for 6 weeks. At the end of the experiment, all animals sacrificed by cervical dislocation. Whole length Small intestine was removed immediately and immersed in 10% buffered formalin. After fixation, 1cm-thick samples were taken from the middle parts of duodenum (from the gizzard to pancreatic and bile duct), jejunum (from the bile duct to Meckel’ s diverticulum) and ileum (from the Meckel’ s diverticulum to ileo-cecal-colonic junction). After routine histological laboratory methods, 6μ m-thick transverse cross-sections were made, a total number of 10 sections used from each intestinal segment of each bird; sections stained with Haematoxylin and Eosin and Periodic acid-Schiff. For measuring length and width of villi, depth of crypts and goblet cells number Axio vixion Rel 4. 8 software were used. Data were expressed as Mean± SD. Data analysis was performed by Independent-Samples T Test method and differences considered statistically significant at p

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APA: Copy

Khalkhali, Ashkan, hamedi, somayeh, & paryani, mohammadreza. (2020). Effect of methionine deficiency on small intestinal histology in Japanese quail. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 13(4 (52) ), 341-352. SID. https://sid.ir/paper/183029/en

Vancouver: Copy

Khalkhali Ashkan, hamedi somayeh, paryani mohammadreza. Effect of methionine deficiency on small intestinal histology in Japanese quail. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2020;13(4 (52) ):341-352. Available from: https://sid.ir/paper/183029/en

IEEE: Copy

Ashkan Khalkhali, somayeh hamedi, and mohammadreza paryani, “Effect of methionine deficiency on small intestinal histology in Japanese quail,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 13, no. 4 (52) , pp. 341–352, 2020, [Online]. Available: https://sid.ir/paper/183029/en

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