STUDY OF POLYMORPHISM OF GROWTH HORMONE-RELEASING HORMONE (GHRH) ALLELES IN IRANIAN SARABI COWS

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KHOSRAVI M.; AMINAFSHAR M.; CHAMANI M.

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Paper Information

Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2013 | Volume:7 | Issue:2 (26) Page(s): 1898-1904

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Title

STUDY OF POLYMORPHISM OF GROWTH HORMONE-RELEASING HORMONE (GHRH) ALLELES IN IRANIAN SARABI COWS

Author(s)

KHOSRAVI M. | AMINAFSHAR M. | CHAMANI M. | Issue Writer Certificate

Keywords

SARABI COWSQ2

GHRHQ2

POLYMORPHISMQ2

Abstract

Selection based on molecular markers is one of the new methods that may improve progress and accuracy of selection in animal breeding programs. The GHRH gene (Growth Hormone-releasing Hormone) is a candidate gene for marker-assisted selection strategies. Polymorphs of GHRH gene are reported to be significantly associated with milk production and constituent traits. In order to study the POLYMORPHISM of GHRH gene, blood samples were collected from 112 SARABI COWS. Genomic DNA was extracted and a fragment of 297 bp in size was amplified using polymerase chain reaction. The amplified fragments were subjected to restriction digestion with HaeIII endonuclease enzyme and the resultant digested products were run on 2% Agarose gel. The results revealed the existence of two alleles of GHRHA and GHRHB for the examined locus with frequencies of 0.19 and 0.81 respectively. Three different genotypic variants including GHRHA GHRHA, GHRHA GHRHB and GHRHB GHRHB were identified with genotypic frequencies of 0.0357, 0.3037 and 0.6607 respectively. The c2 test showed that population is in Hardy-Weinberg equilibrium (P<0.05). These data provide evidence that sarabi cattle breed have a genetic variability, which opens interesting prospects for future selection programs, especially marker-assistant selection.

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APA: Copy

KHOSRAVI, M., AMINAFSHAR, M., & CHAMANI, M.. (2013). STUDY OF POLYMORPHISM OF GROWTH HORMONE-RELEASING HORMONE (GHRH) ALLELES IN IRANIAN SARABI COWS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 7(2 (26)), 1898-1904. SID. https://sid.ir/paper/183125/en

Vancouver: Copy

KHOSRAVI M., AMINAFSHAR M., CHAMANI M.. STUDY OF POLYMORPHISM OF GROWTH HORMONE-RELEASING HORMONE (GHRH) ALLELES IN IRANIAN SARABI COWS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2013;7(2 (26)):1898-1904. Available from: https://sid.ir/paper/183125/en

IEEE: Copy

M. KHOSRAVI, M. AMINAFSHAR, and M. CHAMANI, “STUDY OF POLYMORPHISM OF GROWTH HORMONE-RELEASING HORMONE (GHRH) ALLELES IN IRANIAN SARABI COWS,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 7, no. 2 (26), pp. 1898–1904, 2013, [Online]. Available: https://sid.ir/paper/183125/en

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