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Information Journal Paper

Title

COMPARING RESULTS OF SERUM LEVEL LDL-CHOLESTROL BY ENZYMATIC AND CALCULATED METHODS IN LABAFINEJAD HOSPITAL IN 1383-1384

Pages

  19-24

Keywords

ENZYMATIC METHODS (WAKO)Q4

Abstract

 Background: The studies of Friedwald and colleagues during the past 30 years about serum level LDL - Cholestrol Measurement show that the CALCULATED METHODs for determination of LDL -CHOLESTROL level except of (Tg>400 mg/dl) hypertriglyceridemic, type 3 hyperlipidemic and non fasting patients have acceptable correlation with ultra centrifuge method. Because the calculated LDL -CHOLESTROL depends on the biologic coefficient varriation of total cholestrol, Tg, HDL, LDL, however, analytic evaluation by direct enzymatic method is necessary. The goal of study is comparing of enzymatic method (WAKO) for direct measurement of LDL -CHOLESTROL with calculated Friedwald and Delong methods.Materials and Methods: We investigated 525 non fasting patient's serum by randum sampling of patients who referred to the Labafinejad hospital serum LDL-cholestrol evaluated by enzymatic (WAKO) method with Diagnostic Kit. Then results of direct method compared with CALCULATED METHODs (Delong and Friedwald).The validity of all three methods are evaluated by opspecs charts and sigmametric methods.Results: This study mean age was 52 Intra assays total cv of direct LDL-cholestrol in comparing to Friedwald and Delong methods were 2.6% and 1.6%, respectively. According to the linear regression analysis r value for Friedwald and Delong were 0/901, 0/894 respectively. Total analytical error of enzymatic, Friedwald and Delong were 8/5%, 9/3% and 0/3% respectively.Conclusion: According to the opspecs chart and sigmametric methods, enzymatic method with CALCULATED METHODs had acceptable, correlation. Because of enzymatic method had maximum sigma number, best functional of point in opspecs chart, minimal observation control and no limitation of CALCULATED METHODs, there for this method showed best efficacy and could be replaced to other methods.

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    APA: Copy

    SAFABAKHSH, SH., & MOHAMMADI TORBATI, P.. (2005). COMPARING RESULTS OF SERUM LEVEL LDL-CHOLESTROL BY ENZYMATIC AND CALCULATED METHODS IN LABAFINEJAD HOSPITAL IN 1383-1384. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 10(1 (43)), 19-24. SID. https://sid.ir/paper/18313/en

    Vancouver: Copy

    SAFABAKHSH SH., MOHAMMADI TORBATI P.. COMPARING RESULTS OF SERUM LEVEL LDL-CHOLESTROL BY ENZYMATIC AND CALCULATED METHODS IN LABAFINEJAD HOSPITAL IN 1383-1384. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2005;10(1 (43)):19-24. Available from: https://sid.ir/paper/18313/en

    IEEE: Copy

    SH. SAFABAKHSH, and P. MOHAMMADI TORBATI, “COMPARING RESULTS OF SERUM LEVEL LDL-CHOLESTROL BY ENZYMATIC AND CALCULATED METHODS IN LABAFINEJAD HOSPITAL IN 1383-1384,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 10, no. 1 (43), pp. 19–24, 2005, [Online]. Available: https://sid.ir/paper/18313/en

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