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Information Journal Paper

Title

DETECTION OF MYCOBACTERIUM BOVIS IN CATTLE SUSPECTED OF HAVING BOVINE TUBERCULOSIS IN URMIA BY PCR METHOD

Pages

  773-780

Abstract

 Bovine tuberculosis (TB) is one of the most important zoonotic diseases worldwide. Cases of human tuberculosis of bovine origin have increased in recent years, and this zoonosis has become a public health problem. MYCOBACTERIUM BOVIS, the causative agent of bovine tuberculosis, infects both farm animals and wild mammals, which act as a reservoir for the organism, making it difficult to control the disease. The aim of this study was to detect Mycobaterium bovis in blood and lymph node samples of cattle suspected of having tuberculosis by PCR method. PCR assay amplifies a 500-bp fragment from the M. bovis genome by using the JB21-JB22 primer pair. Samples from 100 cattle suspected of having tuberculosis were initially decontaminated with the petroff method and ziehl – Neelsen staining was performed. Then specimens were checked by microscopic examination for acid fast bacilli and cultured on lowenstein – Jensen medium contaning sodium pyruvate. Specimens were also processed for PCR analysis. DNA was extracted from samples with CTAB and proteinase K method and entered in a PCR reaction using JB21-JB22 primers. Electrophoresis of the PCR products were done on agarose gels and visualized by Etidium Bromide staining. From the 100 suspected animals, 1 (1%) was positive in the ziehl–Neelsen method, 2 (2%) in the microbial culture and 8 (8%) in the PCR method. The results indicated that the JB21-JB22 PCR with high degrees of sensitivity and specificity is the best method for detection of M. bovis in animals affected by bovine tuberculosis.

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    APA: Copy

    SADEGHI ZALI, M.H., FARAJNIA, S., MOADAB, R., KARIMCHANI, H., DELSHAD, R., & JAHANBAKSH, K.. (2010). DETECTION OF MYCOBACTERIUM BOVIS IN CATTLE SUSPECTED OF HAVING BOVINE TUBERCULOSIS IN URMIA BY PCR METHOD. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 4(1 (13)), 773-780. SID. https://sid.ir/paper/183133/en

    Vancouver: Copy

    SADEGHI ZALI M.H., FARAJNIA S., MOADAB R., KARIMCHANI H., DELSHAD R., JAHANBAKSH K.. DETECTION OF MYCOBACTERIUM BOVIS IN CATTLE SUSPECTED OF HAVING BOVINE TUBERCULOSIS IN URMIA BY PCR METHOD. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2010;4(1 (13)):773-780. Available from: https://sid.ir/paper/183133/en

    IEEE: Copy

    M.H. SADEGHI ZALI, S. FARAJNIA, R. MOADAB, H. KARIMCHANI, R. DELSHAD, and K. JAHANBAKSH, “DETECTION OF MYCOBACTERIUM BOVIS IN CATTLE SUSPECTED OF HAVING BOVINE TUBERCULOSIS IN URMIA BY PCR METHOD,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 4, no. 1 (13), pp. 773–780, 2010, [Online]. Available: https://sid.ir/paper/183133/en

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