ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE

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KAZEMI B.; HADADI Z.M.; TAHVILDAR BIDROUNI F.; GHADIRI A.

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Journal: Researcher Bulletin of Medical Sciences Year:2003 | Volume:8 | Issue:3 (33) Page(s): 153-156

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Title

ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE

Author(s)

KAZEMI B. | HADADI Z.M. | TAHVILDAR BIDROUNI F. | GHADIRI A. | Issue Writer Certificate

Keywords

LIZARD LEISHMANIAQ4

ANTIGEN FRACTIONQ4

AMMONIUM SULFATE PRECIPITATIONQ4

GEL FILTERATIONQ4

Abstract

Background: LIZARD LEISHMANIA has different specimen with variety of characteristics. Prior investigators have revealed that some LIZARD LEISHMANIA specimen could immunize balb-C rats against major leishmania. The present study was conducted to isolate a protein fraction of LIZARD LEISHMANIA to be used as vaccine against leishmana. Materials and methods: A species of leishmana promastigote was grown up by inoculation of lizard cardiac blood on NNN medium and irrigated thrice by PBS. In order to prepare a prefect and effective vaccine, we isolated a 68 KDa protein fragment of LIZARD LEISHMANIA promastigote by using AMMONIUM SULFATE PRECIPITATION and gel filtration chromatography techniques.Results: A protein band of almost 68 KDa was observed on AMMONIUM SULFATE PRECIPITATION. Then, it was passed through chromatography column containing Cephadex gel of G100 .Finally, the protein was dialyzed and the antibody was prepared in rabbit.Conclusion: This protein has great antigenicity and could be used to develop a vaccine.

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APA: Copy

KAZEMI, B., HADADI, Z.M., TAHVILDAR BIDROUNI, F., & GHADIRI, A.. (2003). ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 8(3 (33)), 153-156. SID. https://sid.ir/paper/18319/en

Vancouver: Copy

KAZEMI B., HADADI Z.M., TAHVILDAR BIDROUNI F., GHADIRI A.. ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2003;8(3 (33)):153-156. Available from: https://sid.ir/paper/18319/en

IEEE: Copy

B. KAZEMI, Z.M. HADADI, F. TAHVILDAR BIDROUNI, and A. GHADIRI, “ISOLATING A 68 KDA PROTEIN FRACTION OF LIZARD LEISHMANIA PROMASTIGOTE,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 8, no. 3 (33), pp. 153–156, 2003, [Online]. Available: https://sid.ir/paper/18319/en

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