ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH

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KARIMI SH.; AZIZI M.R.

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Paper Information

Journal: Researcher Bulletin of Medical Sciences Year:2003 | Volume:8 | Issue:3 (33) Page(s): 207-211

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Title

ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH

Author(s)

KARIMI SH. | AZIZI M.R. | Issue Writer Certificate

Keywords

ANTIGEN RETRIEVALQ4

IMMUNOHISTOCHEMICAL STAININGQ4

PHQ3

Abstract

Background: ANTIGEN RETRIEVAL (AR) incorporate high temperature heating of tissue sections before immunostaining that strongly increases intensity of IMMUNOHISTOCHEMICAL STAINING.Materials and methods: We have tested the hypothesis that PH of the AR solution may influence the quality of immunostaining by using 3 different AR buffer solutions at 3 different PH values (2.7, 6.16 and 9.27). We have evaluated the staining of 9 antibodies on routinely formalin fixed embedded sections under different PH condition with microwave heating for 10 minutes. The intensity of immounostaining was graded by two pathologists.Results: Antigens have revealed different patterns of staining in different PH. Some antigens showed more or less equal intensity of IMMUNOHISTOCHEMICAL STAINING in these PH. Others showed a dramatic decrease in the intensity of AR IMMUNOHISTOCHEMICAL STAINING at PH=6.16. Still others showed their strongest intensity in PH=9.27.Conclusion: According to several studies concerning optimization of AR system and the aforementioned findings, standardization of AR system should therefore include optimization of the temperature and PH. Meanwhile, PH=9.27 seems to be suitable for most antigens to be effectively retrieved.

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APA: Copy

KARIMI, SH., & AZIZI, M.R.. (2003). ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 8(3 (33)), 207-211. SID. https://sid.ir/paper/18320/en

Vancouver: Copy

KARIMI SH., AZIZI M.R.. ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2003;8(3 (33)):207-211. Available from: https://sid.ir/paper/18320/en

IEEE: Copy

SH. KARIMI, and M.R. AZIZI, “ANTIGEN RETRIEVAL IMMUNOHISTOCHEMISTRY UNDER THE INFLUENCE OF PH,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 8, no. 3 (33), pp. 207–211, 2003, [Online]. Available: https://sid.ir/paper/18320/en

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