EFFECTS OF CYSTEAMINE AND VITAMIN E ADDITION TO SEMEN EXTENDER ON POST-THAWED MICROSCOPIC SPERM PARAMETERS OF BUFFALO BULLS

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BEHESHTI R.; GHIASI GHALEH KANDI J.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ) Year:2014 | Volume:7 | Issue:4 (28) Page(s): 268-276

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Title

EFFECTS OF CYSTEAMINE AND VITAMIN E ADDITION TO SEMEN EXTENDER ON POST-THAWED MICROSCOPIC SPERM PARAMETERS OF BUFFALO BULLS

Author(s)

BEHESHTI R. | GHIASI GHALEH KANDI J. | Issue Writer Certificate

Keywords

SEMEN FREEZINGQ2

BUFFALOQ3

VITAMIN EQ2

CYSTEAMINEQ2

Abstract

This study was designed to investigate the effect of CYSTEAMINE and VITAMIN E on post thaw BUFFALO bull's sperm quality. For this purpose, 20 ejaculates from four BUFFALO bulls possessing more than 70% visual sperm motility were diluted at 37oC in BioXcell® extender. The diluted semen was cooled to 4oC within 2 hours, equilibrated at 4oC following the addition of (0.75, 1.5, 2 and 5 mM) of VITAMIN E and (7.5, 12.5, 15 and 20 mM) CYSTEAMINE per 90 ml, filled in 0.5 ml French straws and were subjected to cooling condition before being plunged into liquid nitrogen. Semen was thawed at 37oC for 40 seconds after 72 hours of storage inside liquid nitrogen .Post-thaw sperm motility and some qualitative parameters of each frozen semen sample were assessed by using computer assisted semen analyzer (CASA). In general, the results showed that the addition of 1.5 mM VITAMIN E and 20 mM CYSTEAMINE in the commercial diluents BioXcell extender for freezing BUFFALO semen increased the motility of spermatozoa and some qualitative parameters to post-thawed BUFFALO sperm.

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APA: Copy

BEHESHTI, R., & GHIASI GHALEH KANDI, J.. (2014). EFFECTS OF CYSTEAMINE AND VITAMIN E ADDITION TO SEMEN EXTENDER ON POST-THAWED MICROSCOPIC SPERM PARAMETERS OF BUFFALO BULLS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), 7(4 (28)), 268-276. SID. https://sid.ir/paper/183251/en

Vancouver: Copy

BEHESHTI R., GHIASI GHALEH KANDI J.. EFFECTS OF CYSTEAMINE AND VITAMIN E ADDITION TO SEMEN EXTENDER ON POST-THAWED MICROSCOPIC SPERM PARAMETERS OF BUFFALO BULLS. VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)[Internet]. 2014;7(4 (28)):268-276. Available from: https://sid.ir/paper/183251/en

IEEE: Copy

R. BEHESHTI, and J. GHIASI GHALEH KANDI, “EFFECTS OF CYSTEAMINE AND VITAMIN E ADDITION TO SEMEN EXTENDER ON POST-THAWED MICROSCOPIC SPERM PARAMETERS OF BUFFALO BULLS,” VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ), vol. 7, no. 4 (28), pp. 268–276, 2014, [Online]. Available: https://sid.ir/paper/183251/en

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