QUANTITATIVE DETECTION OF MAJOR BCR-ABL GENE TRANSCRIPTS BY COMPETITIVE RT-PCR IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAZEMI A.; HASHEMI M.; SHARIFI SH.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN) Year:2008 | Volume:3 | Issue:2 Page(s): 55-62

Download Full-Text

Persian Verion

View:

1,124

Download:

Cites:

Information Journal Paper

Title

QUANTITATIVE DETECTION OF MAJOR BCR-ABL GENE TRANSCRIPTS BY COMPETITIVE RT-PCR IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS

Author(s)

NAZEMI A. | HASHEMI M. | SHARIFI SH. | Issue Writer Certificate

Keywords

COMPETITIVE RT-PCRQ3

INTERNAL CONTROLQ2

BCR/ABL TRANSCRIPTSQ3

Abstract

Chronic Myeloid Leukemia (CML) is produced by the chromosomal translocation and fusion of bcr/abl genes. The purpose of this study was the development of a simple and low-cost technique for quantitative detection of BCR/ABL TRANSCRIPTS in CML patients. This study, we used the COMPETITIVE RT-PCR technique to determine the number of BCR/ABL TRANSCRIPTS. The INTERNAL CONTROL was designed from a non-human DNA source with the same flanking sequences but a smaller size than the target segment. After amplification and purification of INTERNAL CONTROL DNA, PCR products were quantified based on copy numbers. In order to optimize the reaction condition, COMPETITIVE RT-PCRs were carried out separately on target and INTERNAL CONTROL DNAs each with specific copy numbers, and final products of all concentrations were run on agarose gel electrophoresis.The simultaneous reactions of target RNA with different copy numbers of INTERNAL CONTROL DNA showed that the copy numbers of target RNA can be determined by comparison of dye density emission of DNA bands (target and control DNA) on the gel. This study shows that COMPETITIVE RT-PCR is a partially efficient and cheap method for quantitative determination of BCR/ABL TRANSCRIPTS, compared to other methods such as Real-time PCR.

Cites

No record.

References

No record.

Cite

APA: Copy

NAZEMI, A., HASHEMI, M., & SHARIFI, SH.. (2008). QUANTITATIVE DETECTION OF MAJOR BCR-ABL GENE TRANSCRIPTS BY COMPETITIVE RT-PCR IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), 3(2), 55-62. SID. https://sid.ir/paper/183706/en

Vancouver: Copy

NAZEMI A., HASHEMI M., SHARIFI SH.. QUANTITATIVE DETECTION OF MAJOR BCR-ABL GENE TRANSCRIPTS BY COMPETITIVE RT-PCR IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN)[Internet]. 2008;3(2):55-62. Available from: https://sid.ir/paper/183706/en

IEEE: Copy

A. NAZEMI, M. HASHEMI, and SH. SHARIFI, “QUANTITATIVE DETECTION OF MAJOR BCR-ABL GENE TRANSCRIPTS BY COMPETITIVE RT-PCR IN CHRONIC MYELOID LEUKEMIA (CML) PATIENTS,” BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), vol. 3, no. 2, pp. 55–62, 2008, [Online]. Available: https://sid.ir/paper/183706/en

Related Journal Papers