IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SUGAR BEET RHIZOMANIA GENE RESISTANCE USING BULK SEGREGATION ANALYSIS

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FEGHHI S.M.A.; NOROUZI P.; SAIDI A.; ZARGARI K.

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Journal: BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN) Year:2009 | Volume:4 | Issue:1 Page(s): 1-7

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Title

IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SUGAR BEET RHIZOMANIA GENE RESISTANCE USING BULK SEGREGATION ANALYSIS

Author(s)

FEGHHI S.M.A. | NOROUZI P. | SAIDI A. | ZARGARI K. | Issue Writer Certificate

Keywords

RHIZOMANIA DISEASEQ2

RAPD MARKERQ2

PRIMER PAIRSQ3

SUGAR BEETQ2

RZ1 GENEQ3

Abstract

Rhizomania is one of the most important SUGAR BEET diseases in Iran and some other parts of the world, which has an important role in decreasing root yield and sugar content. One approach to combat this disease is the use of resistant varieties. Tagging resistance genes with molecular markers is necessary in breeding programs. In order to identify molecular markers associated with rhizomania resistance gene from the Holly source, a F2 population was developed by researchers at the SUGAR BEET Seed Institute, Karaj, Iran. In this study, pair combinations of 215 RAPD primers were used in bulk segregant analysis (BSA) of two bulks (resistant and susceptible) and F2 population individuals. Three PRIMER PAIRS showed polymorphism in DNA bulks. After testing these PRIMER PAIRS on single plants of the two susceptible and resistant bulks, they were then used to analyze the F2 single plants. Finally, the markers' distance from the resistance gene was estimated by using both the Mapmaker ver.3.0 and the frequency of recombinant plants method. The RZ1 GENE was tagged using R2 marker which was 8.3 cM apart in repulsion phase and C2, C5 and C6 markers which were 32.87, 43.7 and 51.9 cM apart in coupling phase respectively. The data presented is discussed to the possibility of using RAPD MARKERs in SUGAR BEET breeding for resistance to rhizomania.

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APA: Copy

FEGHHI, S.M.A., NOROUZI, P., SAIDI, A., & ZARGARI, K.. (2009). IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SUGAR BEET RHIZOMANIA GENE RESISTANCE USING BULK SEGREGATION ANALYSIS. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), 4(1), 1-7. SID. https://sid.ir/paper/183720/en

Vancouver: Copy

FEGHHI S.M.A., NOROUZI P., SAIDI A., ZARGARI K.. IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SUGAR BEET RHIZOMANIA GENE RESISTANCE USING BULK SEGREGATION ANALYSIS. BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN)[Internet]. 2009;4(1):1-7. Available from: https://sid.ir/paper/183720/en

IEEE: Copy

S.M.A. FEGHHI, P. NOROUZI, A. SAIDI, and K. ZARGARI, “IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SUGAR BEET RHIZOMANIA GENE RESISTANCE USING BULK SEGREGATION ANALYSIS,” BIOLOGICAL SCIENCES (DANISH-I ZISTI-I IRAN), vol. 4, no. 1, pp. 1–7, 2009, [Online]. Available: https://sid.ir/paper/183720/en

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