MECHANISM OF PARAOXON EFFECT ON [3H] GABA UPTAKE INHIBITION IN BRAIN SYNAPTOSOMES OF RAT

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GHASEMI ASGHAR; SADIDI A.; MOHAMMADI MOSLEM; KHOUSHBATEN A.; ASGARI ALI REZA

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Journal: Researcher Bulletin of Medical Sciences Year:2008 | Volume:12 | Issue:6 (60) Page(s): 527-534

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Title

MECHANISM OF PARAOXON EFFECT ON [3H] GABA UPTAKE INHIBITION IN BRAIN SYNAPTOSOMES OF RAT

Author(s)

Keywords

PARAOXONQ3

SYNAPTOSOMEQ3

GABA UPTAKEQ3

VMAXQ3

KMQ4

ORGANOPHOSPHORUS COMPOUNDSQ3

Abstract

Background: Parathion is an organophosphate compound that is frequently used as an insecticide. PARAOXON is the metabolic product of parathion which previously reported that inhibits GABA UPTAKE by rat cerebral cortex SYNAPTOSOMEs. The aim of this study is to investigate possible underlying inhibitory mechanism(s) of PARAOXON on GABA UPTAKE, in SYNAPTOSOMEs prepared from rat cerebral cortex.Materials and Methods: After preparation of SYNAPTOSOMEs, kinetic studies were performed to determe the effect of PARAOXON on Km and Vmax of GABA UPTAKE. Acetylcholine and its antagonists (atropineand mecamylamine) were used to evaluate cholinergic dependency of GABA UPTAKE. Type of GABA transporter involved was determined using beta-alanine and DABA. Results: The results of the study showed that PARAOXON significantly decreased Vmax (175.2±4.23vs. 80.4±2.03 pmol/mg protein/min, P<0.001) of GABA UPTAKE while had no effect on its km (9.80±1.02vs. 9.09±0.92/mM in paradoxes and control groups respectively). DABA significantly decreased GABA UPTAKE (P<0.001) while beta-alanine had no effect. Acetylcholine had no effect on GABA UPTAKE. On the other hand, neither atropine nor mecamylamine could reverse the inhibitory effect of PARAOXON on GABA UPTAKE. Conclusion: In conclusion, it seems that PARAOXON acts as non-competitive antagonist of GABA UPTAKE, which affects kinetics of GABA UPTAKE in nerve endings. We also conclude that the inhibitory effect of PARAOXON on GABA UPTAKE is cholinergic-independent.

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APA: Copy

GHASEMI, ASGHAR, SADIDI, A., MOHAMMADI, MOSLEM, KHOUSHBATEN, A., & ASGARI, ALI REZA. (2008). MECHANISM OF PARAOXON EFFECT ON [³H] GABA UPTAKE INHIBITION IN BRAIN SYNAPTOSOMES OF RAT. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), 12(6 (60)), 527-534. SID. https://sid.ir/paper/18506/en

Vancouver: Copy

GHASEMI ASGHAR, SADIDI A., MOHAMMADI MOSLEM, KHOUSHBATEN A., ASGARI ALI REZA. MECHANISM OF PARAOXON EFFECT ON [³H] GABA UPTAKE INHIBITION IN BRAIN SYNAPTOSOMES OF RAT. RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH)[Internet]. 2008;12(6 (60)):527-534. Available from: https://sid.ir/paper/18506/en

IEEE: Copy

ASGHAR GHASEMI, A. SADIDI, MOSLEM MOHAMMADI, A. KHOUSHBATEN, and ALI REZA ASGARI, “MECHANISM OF PARAOXON EFFECT ON [³H] GABA UPTAKE INHIBITION IN BRAIN SYNAPTOSOMES OF RAT,” RESEARCHER BULLETIN OF MEDICAL SCIENCES (PEJOUHANDEH), vol. 12, no. 6 (60), pp. 527–534, 2008, [Online]. Available: https://sid.ir/paper/18506/en

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