THE EFFECT OF DIFFERENT CONCENTRATIONS OF NAA AND KIN ON MICROPROPAGATION OF MATTHIOLA INCANA

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KAVIANI B.; AHMADI HESAR A.; TARANG A.R.; BOHLOOLI ZANJANI S.; HASHEMABADI D.

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Journal: JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY Year:2013 | Volume:8 | Issue:SPECIAL ISSUE Page(s): 32-39

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Title

THE EFFECT OF DIFFERENT CONCENTRATIONS OF NAA AND KIN ON MICROPROPAGATION OF MATTHIOLA INCANA

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Keywords

GROWTHQ2

MATTHIOLA INCANAQ2

MICROPROPAGATIONQ2

PLANT GROWTH REGULATORSQ3

Abstract

One of the most applications of tissue culture techniques is MICROPROPAGATION. MICROPROPAGATION is an effective technique for propagation of pathogen-free ornamental plants. Type and concentration of plant GROWTH regulators are two important factors in MICROPROPAGATION. Studies on MICROPROPAGATION of MATTHIOLA INCANA, as an ornamental plant, are rare. The aim of this study was to evaluate the effect of naphthalene acetic acid (NAA) and kinetin (KIN) on MICROPROPAGATION of MATTHIOLA INCANA. Seeds from mother plants germinated on MS medium without hormone. Shoot tips from in vitro germinated seedlings were transferred on solid MS medium supplemented with kinetin (KIN) (0, 0.5, 1 and 2 mg/L) and naphthalene acetic acid (NAA) (0, 0.5, 1 and 2 mg/L). The experiment conducted based on completely randomized blocks. MS medium supplemented with 2 mg/L KIN without NAA resulted in the best shoot length and largest number of node. The highest root number was observed in the medium with 2 mg/L NAA without KIN. The longest root was observed in the medium with 1 mg/L NAA+2 mg/L KIN. The highest fresh weight, dry weight and chlorophyll index of plants were observed in the media containing 0.5 mg/L NAA+1 mg/L KIN, 1 mg/L NAA, and 1 mg/L NAA+2 mg/L KIN, respectively. The results of our study showed that the appropriate concentrations of NAA and KIN cause faster propagation of M. incana under tissue culture conditions.

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APA: Copy

KAVIANI, B., AHMADI HESAR, A., TARANG, A.R., BOHLOOLI ZANJANI, S., & HASHEMABADI, D.. (2013). THE EFFECT OF DIFFERENT CONCENTRATIONS OF NAA AND KIN ON MICROPROPAGATION OF MATTHIOLA INCANA. JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, 8(SPECIAL ISSUE), 32-39. SID. https://sid.ir/paper/185615/en

Vancouver: Copy

KAVIANI B., AHMADI HESAR A., TARANG A.R., BOHLOOLI ZANJANI S., HASHEMABADI D.. THE EFFECT OF DIFFERENT CONCENTRATIONS OF NAA AND KIN ON MICROPROPAGATION OF MATTHIOLA INCANA. JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY[Internet]. 2013;8(SPECIAL ISSUE):32-39. Available from: https://sid.ir/paper/185615/en

IEEE: Copy

B. KAVIANI, A. AHMADI HESAR, A.R. TARANG, S. BOHLOOLI ZANJANI, and D. HASHEMABADI, “THE EFFECT OF DIFFERENT CONCENTRATIONS OF NAA AND KIN ON MICROPROPAGATION OF MATTHIOLA INCANA,” JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, vol. 8, no. SPECIAL ISSUE, pp. 32–39, 2013, [Online]. Available: https://sid.ir/paper/185615/en

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