STUDY EFFECTS OF SOME PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS AND ITS HISTOLOGICAL STAGES IN TOMATO (LYCOPERSICUM ESCULENTUM L. VAR. Y)

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MAJD A.; POOR MOHAMMAD FATALI SH.; MIRZAEI M.

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Paper Information

Journal: JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY Year:2011 | Volume:5 | Issue:4 (20) Page(s): 49-56

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Title

STUDY EFFECTS OF SOME PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS AND ITS HISTOLOGICAL STAGES IN TOMATO (LYCOPERSICUM ESCULENTUM L. VAR. Y)

Author(s)

MAJD A. | POOR MOHAMMAD FATALI SH. | MIRZAEI M. | Issue Writer Certificate

Keywords

SOMATIC EMBRYOGENESISQ2

TOMATO; NAA-NAPTHELENE ACETIC ACIDQ2

BAP-6 BENZYL AMINO PURINEQ3

IAA-INDOLE ACETIC ACIDQ3

MS-MURASHIGE AND SKOOGQ2

Abstract

Tomato is a plant of Solanaceae family with scientific name Lycopersicum esculentum Mill (L). var Y, Because of having vitamins and antioxidants, has nutritive and medical importance. With using plant biotechnology in tissue culture and SOMATIC EMBRYOGENESIS, the optimal callugenesis production, and availability of embryogenesis calluses resulted in proliferation of this plant in large scale with desirable way, is possible. In current study, root, hypocotyle, cotyledon, epicotyle, nodes, and leaves explants that obtained from strile seedlings in basic medium MS, were used. Explants were divided to 7-4 mm sections. Results indicated that root and stem in basic medium MS containing (5mgl -1BAP +2 mgl -1IAA), after 3 weeks in dark callus, with formation of embryonoids reached to globular embryo step. In basic medium MS without hormone, after 2 other weeks culture in light, subculture were differentioned to heart embryos. In second subculture, heart embryo transferred to basic medium MS containing 2mgl-1NAA+2 mgl-1IAA, and after 3 weeks other culture in light, diferentioned to torpedo embryos. Subculture of some samples in same medium resulted in forming of leaved. It appeared that increasing amount of auxin causes the roots are formed.

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APA: Copy

MAJD, A., POOR MOHAMMAD FATALI, SH., & MIRZAEI, M.. (2011). STUDY EFFECTS OF SOME PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS AND ITS HISTOLOGICAL STAGES IN TOMATO (LYCOPERSICUM ESCULENTUM L. VAR. Y). JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, 5(4 (20)), 49-56. SID. https://sid.ir/paper/185785/en

Vancouver: Copy

MAJD A., POOR MOHAMMAD FATALI SH., MIRZAEI M.. STUDY EFFECTS OF SOME PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS AND ITS HISTOLOGICAL STAGES IN TOMATO (LYCOPERSICUM ESCULENTUM L. VAR. Y). JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY[Internet]. 2011;5(4 (20)):49-56. Available from: https://sid.ir/paper/185785/en

IEEE: Copy

A. MAJD, SH. POOR MOHAMMAD FATALI, and M. MIRZAEI, “STUDY EFFECTS OF SOME PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS AND ITS HISTOLOGICAL STAGES IN TOMATO (LYCOPERSICUM ESCULENTUM L. VAR. Y),” JOURNAL OF PLANT ENVIRONMENTAL PHYSIOLOGY, vol. 5, no. 4 (20), pp. 49–56, 2011, [Online]. Available: https://sid.ir/paper/185785/en

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