GENETIC DIVERSITY OF POPLAR CLONES USING RANDOM AMPLIFIED POLYMORPHIC (RAPD)DNA

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ASADI FARHAD; NADERI SHAHAB M.A.; MIRZAEI NADOUSHAN HOSSEIN

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Journal: Year:2001 | Volume:14 | Issue:1 (50 IN NATURAL RESOURCES) Page(s): 36-44

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Information Journal Paper

Title

GENETIC DIVERSITY OF POPLAR CLONES USING RANDOM AMPLIFIED POLYMORPHIC (RAPD)DNA

Author(s)

ASADI FARHAD | NADERI SHAHAB M.A. | MIRZAEI NADOUSHAN HOSSEIN | Issue Writer Certificate

Keywords

POPLAR. RAPD. CLONAL IDENTIFICATIONQ2

GENETIC DIVERSITYQ3

DNA FINGERPRINTINGQ4

PCRQ4

PRIMERSQ4

Abstract

Clonal identification of some poplar species is carried out using morphological and phonological characters. This approach does not render a proper differentiation. The goal of this work was to produce molecular patterns which may be useful for clonal identification using the Random P. Amplified Polymorphic DNA (RAP D) technique Twelve poplar clones namely 3 of Populus alba, 3 of P. nigra, 3 of P. euphratica, and 3 of P. deltoides were screened in order to evaluate the GENETIC DIVERSITY of the cited species and clones. Reaction conditions including MgC12 genomic DNA, dNTPs, PRIMERS, Taq DNA polymerase, denaturation, annealing and extension temperature and durations were optimized to get resolved and reproducible band patterns Amplification products were analyzed by electrophoresis in agarose gel and detected by staining with ethidium bromide From the 10 random DNA PRIMERS tested, only PRIMERS including OPN06, OPC04, OPA 16 and DECA 7 were information to differentiate poplar species. Two of the PRIMERS; OPC04 and OPN06 produced polymorphic bands for all clones. The results indicate that the RAPD technique is an adequate tool to obtain DNA fingerprints for clonal identification.

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References

ارزیابی تنوع ژنتیکی موجود در بعضی از توده های اسپرس (Onobrychis sativa) موجود در بانک ژن گیاهان مرتعی ایران

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APA: Copy

ASADI, FARHAD, NADERI SHAHAB, M.A., & MIRZAEI NADOUSHAN, HOSSEIN. (2001). GENETIC DIVERSITY OF POPLAR CLONES USING RANDOM AMPLIFIED POLYMORPHIC (RAPD)DNA. PAJOUHESH-VA-SAZANDEGI, 14(1 (50 IN NATURAL RESOURCES)), 36-44. SID. https://sid.ir/paper/18695/en

Vancouver: Copy

ASADI FARHAD, NADERI SHAHAB M.A., MIRZAEI NADOUSHAN HOSSEIN. GENETIC DIVERSITY OF POPLAR CLONES USING RANDOM AMPLIFIED POLYMORPHIC (RAPD)DNA. PAJOUHESH-VA-SAZANDEGI[Internet]. 2001;14(1 (50 IN NATURAL RESOURCES)):36-44. Available from: https://sid.ir/paper/18695/en

IEEE: Copy

FARHAD ASADI, M.A. NADERI SHAHAB, and HOSSEIN MIRZAEI NADOUSHAN, “GENETIC DIVERSITY OF POPLAR CLONES USING RANDOM AMPLIFIED POLYMORPHIC (RAPD)DNA,” PAJOUHESH-VA-SAZANDEGI, vol. 14, no. 1 (50 IN NATURAL RESOURCES), pp. 36–44, 2001, [Online]. Available: https://sid.ir/paper/18695/en

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