MOLECULAR METHODS FOR DETECTION OF LEPTOSPIRA

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SADAT SAFAVIEH S.; AGHAEIPOUR KH.

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Journal Paper

Paper Information

Journal: Year:2007 | Volume:19 | Issue:1 (74 IN ANIMAL AND FISHERIES SCIENCES) Page(s): 182-188

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Information Journal Paper

Title

MOLECULAR METHODS FOR DETECTION OF LEPTOSPIRA

Author(s)

SADAT SAFAVIEH S. | AGHAEIPOUR KH. | Issue Writer Certificate

Keywords

LEPTOSPIRAQ3

ONA EXTRACTIONQ3

PCRQ3

Abstract

Rapid detection of LEPTOSPIRA is one of necessities in treatment of this zeonostic disease. The unability of serologic methods for detection of these bacteria in initial steps of infection (these methods needs to progress of infection and development of antibody) has candidated molecular methods based on PCR, as an important goal for rapid detection of Leptospirosis. Because of the exiestance of this disease in Iran, it seems a necessity to program for control and treatment of it. For this purpose, the first step is the detection of these bacteria. In this research we developed two PCR methods for detection of the bacteria. Three identified dominant serovars of LEPTOSPIRA in Iran L.canicola, L. sejroe hardjo, L. grippotyphosa were used in development of PCR. The first one was targeted to a non coding locus producting 285bp with G1/G2 set of primers and the second was part of a novel repetitive sequence producing a 571 bp product with primers 590DIR1/590REV2.Comfirmation of PCR product was done with RFLP. AluI and MboI were used for 571 and 285 bp products, respectively.

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References

یافته های تازه پیرامون لپتوسپیروز در موسسه رازی

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APA: Copy

SADAT SAFAVIEH, S., & AGHAEIPOUR, KH.. (2007). MOLECULAR METHODS FOR DETECTION OF LEPTOSPIRA. PAJOUHESH-VA-SAZANDEGI, 19(1 (74 IN ANIMAL AND FISHERIES SCIENCES)), 182-188. SID. https://sid.ir/paper/18786/en

Vancouver: Copy

SADAT SAFAVIEH S., AGHAEIPOUR KH.. MOLECULAR METHODS FOR DETECTION OF LEPTOSPIRA. PAJOUHESH-VA-SAZANDEGI[Internet]. 2007;19(1 (74 IN ANIMAL AND FISHERIES SCIENCES)):182-188. Available from: https://sid.ir/paper/18786/en

IEEE: Copy

S. SADAT SAFAVIEH, and KH. AGHAEIPOUR, “MOLECULAR METHODS FOR DETECTION OF LEPTOSPIRA,” PAJOUHESH-VA-SAZANDEGI, vol. 19, no. 1 (74 IN ANIMAL AND FISHERIES SCIENCES), pp. 182–188, 2007, [Online]. Available: https://sid.ir/paper/18786/en

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