SEQUENCE ANALYSIS OF THE VP2 GENE HYPERVARIABLE REGION OF IRANIAN INFECTIOUS BURSAL DISEASE VIRUSES

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TOROGHI REZA

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Journal: Year:2009 | Volume:21 | Issue:4 (81 IN ANIMAL AND FISHERIES SCIENCES) Page(s): 43-51

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Title

SEQUENCE ANALYSIS OF THE VP2 GENE HYPERVARIABLE REGION OF IRANIAN INFECTIOUS BURSAL DISEASE VIRUSES

Author(s)

TOROGHI REZA | Issue Writer Certificate

Keywords

INFECTIOUS BURSAL DISEASE VIRUSQ3

SEQUENCE ANALYSISQ3

PHYLOGENYQ2

VP2Q3

Abstract

Highy virulent strains of INFECTIOUS BURSAL DISEASE VIRUS (IBDV) emerged in Iran in 1991, devasting Iranian poultry industry. In the present study, molecular characterization of recent Iranian IBD viruses prevalent in the country was carried out. Total 13 IBDV isolates collected from different parts of the country, mostly with the history of high mortality during 2002 to 2003 were used. Highly sensitive techinqe of reverse transcription-polymerase chain reaction (RT-PCR) could detect virus in clinical samples. The 643 bp PCR products generated by amplification of VP2 gene variable region were confirmed by their size in Agarose gel and nested-PCR technique. Deduced amino acid SEQUENCE ANALYSIS of VP2 gene variable region, comprising major virus neutralizing epitopes, revealed Iranian field isolates to be similar to each other and highly virulent IBDV isolates repoted from Europe and Japan. Howerer, most of these viruses could be differentiated from each other at nucleotid level. Out of total nucleotid changes found in Iranian field isolaters four resulted in amino acid changes at positions 222 (P to A), 256 (V to I), 294 (Lto I) and 299 (N to S). Serin rich heptapeptide sequence “SWSASGS” reported to be conserved in pathogenic strains of IBDV was also conserved in Iranian field isolates. None of the field isolates was found to be an antigenically variant or atypical highly virulent IBDV. Phylogenetic analysis revealed that Iraian field isolates originated from the same sourece as that of European and Japanese highly virulent IBDV isolates.

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APA: Copy

TOROGHI, REZA. (2009). SEQUENCE ANALYSIS OF THE VP2 GENE HYPERVARIABLE REGION OF IRANIAN INFECTIOUS BURSAL DISEASE VIRUSES. PAJOUHESH-VA-SAZANDEGI, 21(4 (81 IN ANIMAL AND FISHERIES SCIENCES)), 43-51. SID. https://sid.ir/paper/18846/en

Vancouver: Copy

TOROGHI REZA. SEQUENCE ANALYSIS OF THE VP2 GENE HYPERVARIABLE REGION OF IRANIAN INFECTIOUS BURSAL DISEASE VIRUSES. PAJOUHESH-VA-SAZANDEGI[Internet]. 2009;21(4 (81 IN ANIMAL AND FISHERIES SCIENCES)):43-51. Available from: https://sid.ir/paper/18846/en

IEEE: Copy

REZA TOROGHI, “SEQUENCE ANALYSIS OF THE VP2 GENE HYPERVARIABLE REGION OF IRANIAN INFECTIOUS BURSAL DISEASE VIRUSES,” PAJOUHESH-VA-SAZANDEGI, vol. 21, no. 4 (81 IN ANIMAL AND FISHERIES SCIENCES), pp. 43–51, 2009, [Online]. Available: https://sid.ir/paper/18846/en

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