PRODUCTION OF CARRYING DJ-1 GENE RECOMBINANT LENTIVIRUSES AND THEIR TRANSITION TO HUMAN CELLS

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ESMAEILZADEH E.; GARDANEH M.

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Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2014 | Volume:4 | Issue:4 Page(s): 381-388

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Title

PRODUCTION OF CARRYING DJ-1 GENE RECOMBINANT LENTIVIRUSES AND THEIR TRANSITION TO HUMAN CELLS

Author(s)

ESMAEILZADEH E. | GARDANEH M. | Issue Writer Certificate

Keywords

CLONINGQ2

DJ1Q2

LENTIVIRUSQ2

Abstract

Aim: The aims of this study were subCLONING of Dj-1 (PARK7) gene to lentiviral transfer vector, recombinant LENTIVIRUSes production and target cells infection with these viruses.Material and methods: DJ1 gene obtained from pcDNA-DJ1 vector using restriction enzymes EcoR1 and Xho1. Lentiviral transfer vector was coincidental digested using EcoR1 and Sall enzymes. DJ-1 gene was interred into the lentiviral transfer and upstream of Jred gene using T4-DNA ligase. As DJ1-IRES-Jred sequence was placed in downstream and control of CMV promoter. To production of recombinant lentivruses, the made vector is coincidental transferred into the HEK-293T (Human Embryonic Kidney) cells with two packaging and envelope lentiviral vectors. Produced virus was used for target cells infection.Results: For integrity of subCLONING, enzymatic tests and PCR were used.Fluorescent microscopy also used to show expression of Jred reporter gene that showed the success of our gene transferring. Then RT-PCR was done for showing overexpression of DJ1 gene in transduced cells compare with normal cells.Conclusion: This study show the lentiviral vectors success in gene transferring to eukaryotic cells and clear that this vectors can be used in treatment of nervous system diseases.

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APA: Copy

ESMAEILZADEH, E., & GARDANEH, M.. (2014). PRODUCTION OF CARRYING DJ-1 GENE RECOMBINANT LENTIVIRUSES AND THEIR TRANSITION TO HUMAN CELLS. JOURNAL OF CELL & TISSUE, 4(4), 381-388. SID. https://sid.ir/paper/188922/en

Vancouver: Copy

ESMAEILZADEH E., GARDANEH M.. PRODUCTION OF CARRYING DJ-1 GENE RECOMBINANT LENTIVIRUSES AND THEIR TRANSITION TO HUMAN CELLS. JOURNAL OF CELL & TISSUE[Internet]. 2014;4(4):381-388. Available from: https://sid.ir/paper/188922/en

IEEE: Copy

E. ESMAEILZADEH, and M. GARDANEH, “PRODUCTION OF CARRYING DJ-1 GENE RECOMBINANT LENTIVIRUSES AND THEIR TRANSITION TO HUMAN CELLS,” JOURNAL OF CELL & TISSUE, vol. 4, no. 4, pp. 381–388, 2014, [Online]. Available: https://sid.ir/paper/188922/en

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