EFFECT OF HYDROGEN PEROXIDE ON INDUCTION OF SECONDARY METABOLITES IN PERIWINKLE (CATHARANTHUS ROSEUS L.) CALLUS

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KESHAVARZ R.; MAHDIYEH MAJID; ABNOSI M.H.; AMIRJANI M.R.

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Information Journal Paper

Title

EFFECT OF HYDROGEN PEROXIDE ON INDUCTION OF SECONDARY METABOLITES IN PERIWINKLE (CATHARANTHUS ROSEUS L.) CALLUS

Author(s)

KESHAVARZ R. | MAHDIYEH MAJID | ABNOSI M.H. | AMIRJANI M.R. | Issue Writer Certificate

Keywords

ALKALOIDSQ2

ANTIOXIDANT CALLUSQ2

CATHARANTHUS ROSEUSQ2

FLAVONOIDSQ2

HYDROGEN PEROXIDEQ1

PHENOLSQ1

Abstract

Aim: In the present study the effect of oxidative stress resulted from HYDROGEN PEROXIDE on some secondary metabolites and changes on intra cellular HYDROGEN PEROXIDE has been explored.Material and methods: To induction of callus Periwinkle leaf was sterilized and put on MS medium. Calluses were treated by different concentrations (1, 5 and 10 mM) of HYDROGEN PEROXIDE for 6 days. Total alkaloid, phenolic components and felavonoids as well as antioxidant potential on the basis of Iron reduction were determined. Data were analyzed using one way ANOVA.Results: Addition of HYDROGEN PEROXIDE resulted in significant (P£0.05) increase of intra cellular HYDROGEN PEROXIDE compared to the control. Total ALKALOIDS, FLAVONOIDS and PHENOLS content showed that HYDROGEN PEROXIDE caused a significant increase (P <0.05. Furthermore, HYDROGEN PEROXIDE increased the antioxidant capacity. Better antioxidant capacity was Iron reduction dependent.Conclusion: HYDROGEN PEROXIDE increased intra cellular levels of HYDROGEN PEROXIDE. Secondary metabolites such as ALKALOIDS and phenolic compounds and total flavonoid were boosted as well. Therefore tensions may be applied to increase them.

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APA: Copy

KESHAVARZ, R., MAHDIYEH, MAJID, ABNOSI, M.H., & AMIRJANI, M.R.. (2015). EFFECT OF HYDROGEN PEROXIDE ON INDUCTION OF SECONDARY METABOLITES IN PERIWINKLE (CATHARANTHUS ROSEUS L.) CALLUS. JOURNAL OF CELL & TISSUE, 6(3), 389-396. SID. https://sid.ir/paper/189078/en

Vancouver: Copy

KESHAVARZ R., MAHDIYEH MAJID, ABNOSI M.H., AMIRJANI M.R.. EFFECT OF HYDROGEN PEROXIDE ON INDUCTION OF SECONDARY METABOLITES IN PERIWINKLE (CATHARANTHUS ROSEUS L.) CALLUS. JOURNAL OF CELL & TISSUE[Internet]. 2015;6(3):389-396. Available from: https://sid.ir/paper/189078/en

IEEE: Copy

R. KESHAVARZ, MAJID MAHDIYEH, M.H. ABNOSI, and M.R. AMIRJANI, “EFFECT OF HYDROGEN PEROXIDE ON INDUCTION OF SECONDARY METABOLITES IN PERIWINKLE (CATHARANTHUS ROSEUS L.) CALLUS,” JOURNAL OF CELL & TISSUE, vol. 6, no. 3, pp. 389–396, 2015, [Online]. Available: https://sid.ir/paper/189078/en

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