EVALUATION OF TOXICITY AND ANTICANCER ACTIVITY OF ISOLATED FRACTION FROM THE VENOM OF IRANIAN COBRA SNAKE ON ACUTE LYMPHOBLASTIC LEUKEMIA CELLS (JURKAT E6.1)

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BARATI M.; DAVOUDI DAHAGHANI F.

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Journal: JOURNAL OF CELL & TISSUE Year:2017 | Volume:8 | Issue:3 Page(s): 250-260

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Title

EVALUATION OF TOXICITY AND ANTICANCER ACTIVITY OF ISOLATED FRACTION FROM THE VENOM OF IRANIAN COBRA SNAKE ON ACUTE LYMPHOBLASTIC LEUKEMIA CELLS (JURKAT E6.1)

Author(s)

BARATI M. | DAVOUDI DAHAGHANI F. | Issue Writer Certificate

Keywords

IRANIAN COBRA SNAKE VENOMQ1

LEUKEMIAQ2

MTT ASSAYQ2

Abstract

Aim: Cancer is a life threatening disease determined by uncontrolled differentiation and proliferation of cells. Chemotherapy is the conventional method in treatment of cancer, but the tumor often will be resistant to chemotherapy regimen leading to crucial side effects. Many bio-toxins are biologically active compounds with anti-tumor activity. This study was aimed to find an anti-cancer agent from the venom of Iranian cobra snake.Material and methods: The process of collection, lyophilisation of Iranian cobra (Naja naja oxiana) venom. Protein concentration of Iranian cobra venom was determined by BCA assay. Fast protein liquid chromatography (FPLC), SDS-PAGE, Anion exchange chromatography were used for purification of Iranian cobra venom. Standard cell biology methods were employed to characterize Iranian cobra venom abilities (in vitro) to inhibit Platelet aggregation, adhesion, migration and invasion of tumor cells. Its anti-cancer activity in (Jurkat E6.1) (in vitro) was tested by MTT ASSAY.Results: Peak 3 of FPLC was active on cell lines and selected for anion exchange chromatography. Four anionic and one cationic fractions collected for anticancer activity. Peak 2 of anion exchange chromatography had the most toxic activity as 43%±1 at 1.5 micrograms. This fraction showed about 8%±1 toxicity on fibroblast cells. (P-value ≤ 0.05)Conclusion: Natural anti-cancer compounds derived from the Naja naja oxiana venom, can fight against cancer. It is the first report of an anticancer fraction from Iranian cobra with toxicity on Jurkat E6.1 cell line with less toxicity to normal cells.

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APA: Copy

BARATI, M., & DAVOUDI DAHAGHANI, F.. (2017). EVALUATION OF TOXICITY AND ANTICANCER ACTIVITY OF ISOLATED FRACTION FROM THE VENOM OF IRANIAN COBRA SNAKE ON ACUTE LYMPHOBLASTIC LEUKEMIA CELLS (JURKAT E6.1). JOURNAL OF CELL & TISSUE, 8(3 ), 250-260. SID. https://sid.ir/paper/189087/en

Vancouver: Copy

BARATI M., DAVOUDI DAHAGHANI F.. EVALUATION OF TOXICITY AND ANTICANCER ACTIVITY OF ISOLATED FRACTION FROM THE VENOM OF IRANIAN COBRA SNAKE ON ACUTE LYMPHOBLASTIC LEUKEMIA CELLS (JURKAT E6.1). JOURNAL OF CELL & TISSUE[Internet]. 2017;8(3 ):250-260. Available from: https://sid.ir/paper/189087/en

IEEE: Copy

M. BARATI, and F. DAVOUDI DAHAGHANI, “EVALUATION OF TOXICITY AND ANTICANCER ACTIVITY OF ISOLATED FRACTION FROM THE VENOM OF IRANIAN COBRA SNAKE ON ACUTE LYMPHOBLASTIC LEUKEMIA CELLS (JURKAT E6.1),” JOURNAL OF CELL & TISSUE, vol. 8, no. 3 , pp. 250–260, 2017, [Online]. Available: https://sid.ir/paper/189087/en

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