SCLEROTOMAL DIFFERENTIATION OF SOMITIC CELLS CO-CULTURED WITH CHICKEN EMBRYONIC NOTOCHORD

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RAHBARI R.; MAZANI M.; GOL MOHAMMADI M.GH.; SAGHA MOHSEN

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Journal: JOURNAL OF CELL & TISSUE Year:2014 | Volume:5 | Issue:1 Page(s): 71-77

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Title

SCLEROTOMAL DIFFERENTIATION OF SOMITIC CELLS CO-CULTURED WITH CHICKEN EMBRYONIC NOTOCHORD

Author(s)

RAHBARI R. | MAZANI M. | GOL MOHAMMADI M.GH. | SAGHA MOHSEN | Issue Writer Certificate

Keywords

CHICK EMBRYOQ2

COQ2

CULTUREQ2

NOTOCHORDQ2

SOMITEQ2

Abstract

Aim: The SOMITEs are epithelial blocks of mesodermal cells differentiating to sclerotome and dermomyotome and NOTOCHORD or axial mesoderm plays a major role in somitic cell differentiation to sclerotome. This study was aimed to evaluate the role of the NOTOCHORD in sclerotomal differentiation of the SOMITEs in vitro.Material and Methods: In this experimental study, isolated NOTOCHORDs from CHICK EMBRYO were encapsulated in alginate beads and CO-cultured with SOMITEs for six days. In COntrol group, the SOMITEs were CULTUREd alone. Newly isolated and non-cultured SOMITEs were COnsidered as SOMITEs on day 0. Eventually, morphology of CULTUREd somitic cells was evaluated by inverted microscope and then RT-PCR method was used to analyze the sclerotomal and dermomyotomal gene expression profile.Results: In COmparison with COntrol group, the somitic cells CO-cultured with NOTOCHORD had highly differentiation potential and showed mesenchymal morphology with numerous slender processes. Gene expression profile of CO-cultured somitic cells indicated upregulation of Pax1 and BMP4 and downregulation of MyoD, presenting sclerotomal cell characteristics.Conclusion: Embryonic NOTOCHORD can induce in vitro sclerotomal differentiation in SOMITEs through upregulation of genes involving in this differentiation.

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APA: Copy

RAHBARI, R., MAZANI, M., GOL MOHAMMADI, M.GH., & SAGHA, MOHSEN. (2014). SCLEROTOMAL DIFFERENTIATION OF SOMITIC CELLS CO-CULTURED WITH CHICKEN EMBRYONIC NOTOCHORD. JOURNAL OF CELL & TISSUE, 5(1), 71-77. SID. https://sid.ir/paper/189101/en

Vancouver: Copy

RAHBARI R., MAZANI M., GOL MOHAMMADI M.GH., SAGHA MOHSEN. SCLEROTOMAL DIFFERENTIATION OF SOMITIC CELLS CO-CULTURED WITH CHICKEN EMBRYONIC NOTOCHORD. JOURNAL OF CELL & TISSUE[Internet]. 2014;5(1):71-77. Available from: https://sid.ir/paper/189101/en

IEEE: Copy

R. RAHBARI, M. MAZANI, M.GH. GOL MOHAMMADI, and MOHSEN SAGHA, “SCLEROTOMAL DIFFERENTIATION OF SOMITIC CELLS CO-CULTURED WITH CHICKEN EMBRYONIC NOTOCHORD,” JOURNAL OF CELL & TISSUE, vol. 5, no. 1, pp. 71–77, 2014, [Online]. Available: https://sid.ir/paper/189101/en

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