MICROPROPAGATION, ORGANOGENESIS AND USING NEW METHOD OF SEMIPHOTOAUTOTROPHIC IN EUCALYPTUS GONGYLOCARPA

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ASAREH M.H.; GHORBANLI MAH LAGHA; AKBARI KHABAZ M.; GHAMARI ZARE A.; EMAM MITRA

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Journal: Year:2007 | Volume:20 | Issue:2 (75 IN NATURAL RESOURCES) Page(s): 134-145

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Title

MICROPROPAGATION, ORGANOGENESIS AND USING NEW METHOD OF SEMIPHOTOAUTOTROPHIC IN EUCALYPTUS GONGYLOCARPA

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Keywords

MICROPROPAGATIONQ2

ORGANOGENESISQ3

SEMIPHOTOAUTOTROPHICQ3

EUCALYPTUSQ3

ROOTING AND GROWTH REGULATORQ3

Abstract

MICROPROPAGATION of E. gongylocarpa through bud culture, callus and semiphotoautotropic conditions was investigated. The effects of sterilization, growth regulator treatments and different semi-photoautoautotropic conditions on establishment and multiplication of explants were studied. The best sterilization treatment for seeds was observed under 0.5% Benomyl solution for 15 min, washing with detergent and then under running water for two hours, 70% ethanol for 20 seconds and 2% NaOCl for 20 min. Terminal and auxiliary buds of seedlings after washing with detergent and dipping in acid ascorbic (AS) were sterilized by dipping in 0.1% HgCl2 solution for one and six min, respectively. Terminal and auxiliary buds of adult trees sterilized with 0.1% HgCl2 solution for two and six min, respectively. MS medium containing half strength of nitrate, 0.1 mgl-1 BAP, 0.2 mgl-1 Kin, 0.01 mgl-1 IBA and 0.25 mgl-1 GA3 were selected for shoot establishment and multiplication. The best rhyzogenese, observed in MS medium supplemented with 1 mgl-1 IBA. Enough amount of callus got induced explants on MS medium fortified with 1 mgl-1 Kin and 2 mgl-1 2, 4-D. The most shoot formation occurred on the callus when cultured on MS medium containing 0.5 mgl-1 both of BAP and IBA growth regulators. Three experiments were carried out to elucidate the influence of different culture conditions and environmental factors on growth of E. gongylocarpa. The in vitro growth rate of plantlets was the highest in vermiculite treatment.

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APA: Copy

ASAREH, M.H., GHORBANLI, MAH LAGHA, AKBARI KHABAZ, M., GHAMARI ZARE, A., & EMAM, MITRA. (2007). MICROPROPAGATION, ORGANOGENESIS AND USING NEW METHOD OF SEMIPHOTOAUTOTROPHIC IN EUCALYPTUS GONGYLOCARPA. PAJOUHESH-VA-SAZANDEGI, 20(2 (75 IN NATURAL RESOURCES)), 134-145. SID. https://sid.ir/paper/18911/en

Vancouver: Copy

ASAREH M.H., GHORBANLI MAH LAGHA, AKBARI KHABAZ M., GHAMARI ZARE A., EMAM MITRA. MICROPROPAGATION, ORGANOGENESIS AND USING NEW METHOD OF SEMIPHOTOAUTOTROPHIC IN EUCALYPTUS GONGYLOCARPA. PAJOUHESH-VA-SAZANDEGI[Internet]. 2007;20(2 (75 IN NATURAL RESOURCES)):134-145. Available from: https://sid.ir/paper/18911/en

IEEE: Copy

M.H. ASAREH, MAH LAGHA GHORBANLI, M. AKBARI KHABAZ, A. GHAMARI ZARE, and MITRA EMAM, “MICROPROPAGATION, ORGANOGENESIS AND USING NEW METHOD OF SEMIPHOTOAUTOTROPHIC IN EUCALYPTUS GONGYLOCARPA,” PAJOUHESH-VA-SAZANDEGI, vol. 20, no. 2 (75 IN NATURAL RESOURCES), pp. 134–145, 2007, [Online]. Available: https://sid.ir/paper/18911/en

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