DETECTION OF MYCOBACTERIUM PARATUBERCULOSIS BY NESTED PCR IN DAIRY CATTLES SUSPECTED TO JOHN'S DISEASE

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DOOSTI A.; MOSHKELANI S.

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Journal: JOURNAL OF MICROBIAL WORLD Year:2009 | Volume:2 | Issue:1 Page(s): 19-22

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Information Journal Paper

Title

DETECTION OF MYCOBACTERIUM PARATUBERCULOSIS BY NESTED PCR IN DAIRY CATTLES SUSPECTED TO JOHN'S DISEASE

Author(s)

DOOSTI A. | MOSHKELANI S. | Issue Writer Certificate

Keywords

MYCOBACTERIUM PARATUBERCULOSISQ3

NESTED-PCRQ3

JOHN'S DISEASEQ2

DAIRY CATTLESQ3

Abstract

Background and Objectives: Johne's disease is a chronic intestinal disease of domestic and wild ruminants that is caused by MYCOBACTERIUM PARATUBERCULOSIS. So that MYCOBACTERIUM PARATUBERCULOSIS is a widespread zoonotic disease. It is necessary to prevent it and the elimination of its agent needs the attention of corresponding managers. The aim of this study is based on the use of Nested PCR as an accurate and rapid method to detection of MYCOBACTERIUM PARATUBERCULOSIS bovine feces.Material and Methods: Fecal samples from 120 dairy cattle were collected and DNA extraction was performed from the samples. Then, samples were evaluated with specific primers for the MYCOBACTERIUM PARATUBERCULOSIS-specific IS900 gene by NESTED-PCR assay. Finally, PCR products were electrophoresis on agarose gel.Results: In total, from 120 samples were enrolled in this study, 22 specimen (18.33%) were found positive on the basis of NESTED-PCR analysis.Conclusion: Nested PCR technique is considered as a fast, reliable and affordable assay to detect MYCOBACTERIUM PARATUBERCULOSIS.

Cites

PHYLOGENETIC ANALYSIS OF MYCOBACTERIUM AVIUM SUBSPECIES PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLE OF TEHRAN PROVINCE

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APA: Copy

DOOSTI, A., & MOSHKELANI, S.. (2009). DETECTION OF MYCOBACTERIUM PARATUBERCULOSIS BY NESTED PCR IN DAIRY CATTLES SUSPECTED TO JOHN'S DISEASE. JOURNAL OF MICROBIAL WORLD, 2(1), 19-22. SID. https://sid.ir/paper/189160/en

Vancouver: Copy

DOOSTI A., MOSHKELANI S.. DETECTION OF MYCOBACTERIUM PARATUBERCULOSIS BY NESTED PCR IN DAIRY CATTLES SUSPECTED TO JOHN'S DISEASE. JOURNAL OF MICROBIAL WORLD[Internet]. 2009;2(1):19-22. Available from: https://sid.ir/paper/189160/en

IEEE: Copy

A. DOOSTI, and S. MOSHKELANI, “DETECTION OF MYCOBACTERIUM PARATUBERCULOSIS BY NESTED PCR IN DAIRY CATTLES SUSPECTED TO JOHN'S DISEASE,” JOURNAL OF MICROBIAL WORLD, vol. 2, no. 1, pp. 19–22, 2009, [Online]. Available: https://sid.ir/paper/189160/en

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