EVALUATION OF INVERTASE ACTIVITY IN YEASTS ISOLATED FROM SALINE SOIL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SALEHI SAREH; POURBABAEE AHMAD ALI; REZAEI ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MICROBIAL WORLD Year:2010 | Volume:3 | Issue:1 (6) Page(s): 31-39

Download Full-Text

Persian Verion

View:

2,251

Download:

Cites:

Information Journal Paper

Title

EVALUATION OF INVERTASE ACTIVITY IN YEASTS ISOLATED FROM SALINE SOIL

Author(s)

SALEHI SAREH | POURBABAEE AHMAD ALI | REZAEI ALI | Issue Writer Certificate

Keywords

INVERTASEQ3

FOOD INDUSTRIESQ2

HALOPHILIC YEASTSQ3

HALOTOLERANT YEASTSQ3

Abstract

Background and objective: Biodiversity of yeasts in extreme habitats and applications in food industry attract many of researchers. The aim of this study was evaluation of INVERTASE activity in isolated yeasts from saline soil.Material and Methods: In this study, kinetic activity of INVERTASE from 27 halophilic and halotolerant yeast strains isolated from salin soil of Qom and Arak were evaluated. After collection of soil samples, the yeasts were cultured on YPD Agar containing 5% salt. Out of 27 yeast strains, 2 halophilic and 6 halotolerant strains with INVERTASE activity were assessed for selection of best strain.Results: Assessment of INVERTASE activity according to the Glucose Oxidase assay showed that the enzyme productivity of G5 strain is more powerful than other isolates. The optimum enzyme activity was obtained at 65oC and pH 4.5. The highest enzymatic activity was observed in the medium containing 1.7mM sodium chloride. Na+, Mg2+ and Ca2+ ions (all chlorides and 5mM concentration) have stimulation effect on enzyme's activity. The Km of enzyme for sucrose was 20 mM. Thermal stability of enzyme in 65oC was one hour and decrease of activity is not observed.Conclusion: According to the results of this study, G5 strain has able to produce INVERTASE in different concentrations of different salts.

Cites

No record.

References

No record.

Cite

APA: Copy

SALEHI, SAREH, POURBABAEE, AHMAD ALI, & REZAEI, ALI. (2010). EVALUATION OF INVERTASE ACTIVITY IN YEASTS ISOLATED FROM SALINE SOIL. JOURNAL OF MICROBIAL WORLD, 3(1 (6)), 31-39. SID. https://sid.ir/paper/189284/en

Vancouver: Copy

SALEHI SAREH, POURBABAEE AHMAD ALI, REZAEI ALI. EVALUATION OF INVERTASE ACTIVITY IN YEASTS ISOLATED FROM SALINE SOIL. JOURNAL OF MICROBIAL WORLD[Internet]. 2010;3(1 (6)):31-39. Available from: https://sid.ir/paper/189284/en

IEEE: Copy

SAREH SALEHI, AHMAD ALI POURBABAEE, and ALI REZAEI, “EVALUATION OF INVERTASE ACTIVITY IN YEASTS ISOLATED FROM SALINE SOIL,” JOURNAL OF MICROBIAL WORLD, vol. 3, no. 1 (6), pp. 31–39, 2010, [Online]. Available: https://sid.ir/paper/189284/en

Related Journal Papers