GENOTYPIC OF CLOSTRIDIUM PERFRINGENS IN COLD AREA OF KERMAN PROVINCE

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KHEIRKHAH BABAK; HATAM JAHROMI MARYAM

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Journal: JOURNAL OF MICROBIAL WORLD Year:2016 | Volume:9 | Issue:2 (27) Page(s): 169-175

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Information Journal Paper

Title

GENOTYPIC OF CLOSTRIDIUM PERFRINGENS IN COLD AREA OF KERMAN PROVINCE

Author(s)

KHEIRKHAH BABAK | HATAM JAHROMI MARYAM | Issue Writer Certificate

Keywords

CLOSTRIDIUM PERFRINGENSQ1

ENTEROTOXEMIAQ1

MULTIPLEX-PCRQ2

Abstract

CLOSTRIDIUM PERFRINGENSis an anaerobic, Gram-positive, toxin-forming bacterium and the etiologic cause of enterotoxaemia in animals. Soil is considered as the main source of spore of this bacteria. This study was aimed to genotyping the C. perfringens isolated from intestines of sheep with ENTEROTOXEMIA and as well as from the soil samples collected from cold area of Kerman province using Multiplex- PCR. This cross-sectional study was carried out on 50 soil samples and 50 intestinal samples collected from three cities located at Kerman to isolate the C. perfringens isolates. The C. perfringens isolates were identified based on enrichment of the samples and growing up the bacteria in specific media and following performance of confirmation tests using biochemical tests. A PCR reaction were performed to confirm the presence of these strains. Overall 42 Clostridial strains were isolated in this study, among them 27 isolates were collected from intestinal samples. Also, 10 soil samples were contaminated with Clostridia, among them 5 samples belonged to C. perfringens. This study showed that C. perfringens is the most prevalent species in the sick sheep and soil in comparison to other species. Furthermore, Type D of this species was more prevalent than others.

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APA: Copy

KHEIRKHAH, BABAK, & HATAM JAHROMI, MARYAM. (2016). GENOTYPIC OF CLOSTRIDIUM PERFRINGENS IN COLD AREA OF KERMAN PROVINCE. JOURNAL OF MICROBIAL WORLD, 9(2 (27)), 169-175. SID. https://sid.ir/paper/189362/en

Vancouver: Copy

KHEIRKHAH BABAK, HATAM JAHROMI MARYAM. GENOTYPIC OF CLOSTRIDIUM PERFRINGENS IN COLD AREA OF KERMAN PROVINCE. JOURNAL OF MICROBIAL WORLD[Internet]. 2016;9(2 (27)):169-175. Available from: https://sid.ir/paper/189362/en

IEEE: Copy

BABAK KHEIRKHAH, and MARYAM HATAM JAHROMI, “GENOTYPIC OF CLOSTRIDIUM PERFRINGENS IN COLD AREA OF KERMAN PROVINCE,” JOURNAL OF MICROBIAL WORLD, vol. 9, no. 2 (27), pp. 169–175, 2016, [Online]. Available: https://sid.ir/paper/189362/en

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