ASEXUAL REGENERATION OF MATURE JUGLANS REGIA BY SHOOT TIP CULTURE

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EMAM M.

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Journal: Year:2004 | Volume:17 | Issue:(63 IN NATURAL RESOURCES) Page(s): 10-15

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Information Journal Paper

Title

ASEXUAL REGENERATION OF MATURE JUGLANS REGIA BY SHOOT TIP CULTURE

Author(s)

EMAM M. | Issue Writer Certificate

Keywords

TISSUE CULTUREQ4

JUGLANS REGIAQ3

SHOOTTIP CULTUREQ3

Abstract

In order to investigate asexual reproduction of walnut (Juglans regia), shoot tips and axillary buds of elite adult trees were collected from natural stands and used as explants. Suitable method of sterilization was found.That is about dipping the explants in ethanol solution(70%) for I minutes and using of Mercuric chloride solution (0.1%) for 1.5 minutes. Sterilized explants cultured on media with different concentration at different growth regulators. DKW medium containing 1mg/l of BA was the best medium for shoot proliferation. GA3, promoted latheral bud growth and shoot proliferation. MS and DKW medium containing 0.25 mg/lit GA3 and 0.5 mg/lit BA, were the best composition for shoot proliferation. Shoots cultured on hormone free DKW medium before rooting treatments, were longer and more vigorous. In spite of applying different treatments(like decreasing macroelements and sucrose concentrates, using of activated charcole and adding different vitamins and aminoacids to the medium) root development of proliferated shoots were problematic in this species.

Cites

اثر دو نوع محیط کشت، اسید جیبرلیک و چند فاکتور فیزیکی بر جوانه زنی جنین های گردوی ایرانی (Juglans regia L)

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APA: Copy

EMAM, M.. (2004). ASEXUAL REGENERATION OF MATURE JUGLANS REGIA BY SHOOT TIP CULTURE. PAJOUHESH-VA-SAZANDEGI, 17((63 IN NATURAL RESOURCES)), 10-15. SID. https://sid.ir/paper/19103/en

Vancouver: Copy

EMAM M.. ASEXUAL REGENERATION OF MATURE JUGLANS REGIA BY SHOOT TIP CULTURE. PAJOUHESH-VA-SAZANDEGI[Internet]. 2004;17((63 IN NATURAL RESOURCES)):10-15. Available from: https://sid.ir/paper/19103/en

IEEE: Copy

M. EMAM, “ASEXUAL REGENERATION OF MATURE JUGLANS REGIA BY SHOOT TIP CULTURE,” PAJOUHESH-VA-SAZANDEGI, vol. 17, no. (63 IN NATURAL RESOURCES), pp. 10–15, 2004, [Online]. Available: https://sid.ir/paper/19103/en

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