EVALUATION OF THE EFFECTS OF A CELL PERMEABLE ANTIOXIDANT ON MOTILITY AND MEMBRANE INTEGRITY DURING LIQUID STORAGE OF GOAT SEMEN

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ABID A.; FOROZANFAR M.; HOSEINI M.; NASRE ESFEHANI M.

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Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES) Year:2013 | Volume:6 | Issue:1 (20) Page(s): 19-28

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Title

EVALUATION OF THE EFFECTS OF A CELL PERMEABLE ANTIOXIDANT ON MOTILITY AND MEMBRANE INTEGRITY DURING LIQUID STORAGE OF GOAT SEMEN

Author(s)

ABID A. | FOROZANFAR M. | HOSEINI M. | NASRE ESFEHANI M. | Issue Writer Certificate

Keywords

GOAT SPERMQ2

ANTIOXIDANTQ1

MNTEQ2

FREE RADICALSQ2

Abstract

Introduction & Objective: present study was aimed to evaluate the effects of addition of a synthetic ANTIOXIDANT, MNTE, on motility and membrane integrity during the liquid storage (up to 72h) of goat semen.Materials and Methods: Semen samples were collected using artificial vagina from three mature goats, mixed and diluted (1:20 v/v) with a Bioxell extender containing 0 (control), 0.01, 0.1 and 1 mM of MNTE. All diluted sperm suspensions were cooled to 5oC and stored until 72hr and sperm motility and membrane integrity were assessed in 0, 12, 24, 36, 48 and 72hr post dilution. Results: With increasing storage time (0 to 72h), the percentage of motility and membrane integrity decreased in all treatments and control group. Addition of MNTE at 0.01 and 0.1 mM concentration to the extender, improved goat semen quality, while addition of 1mM of MNTE had an adverse effect on motility and membrane integrity when compared to control.Conclusion: extender supplemented with MNTE could reduce the oxidative stress provoked by liquid storage processes. The optimum concentration of MNTE seems to be 0.01 and 0.1mM for goat semen in liquid storage state. However, further supplementation of extender with 1mM of MNTE not only did not improve the results but inversely affected motility and membrane integrity in all treatment group.

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APA: Copy

ABID, A., FOROZANFAR, M., HOSEINI, M., & NASRE ESFEHANI, M.. (2013). EVALUATION OF THE EFFECTS OF A CELL PERMEABLE ANTIOXIDANT ON MOTILITY AND MEMBRANE INTEGRITY DURING LIQUID STORAGE OF GOAT SEMEN. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), 6(1 (20)), 19-28. SID. https://sid.ir/paper/191753/en

Vancouver: Copy

ABID A., FOROZANFAR M., HOSEINI M., NASRE ESFEHANI M.. EVALUATION OF THE EFFECTS OF A CELL PERMEABLE ANTIOXIDANT ON MOTILITY AND MEMBRANE INTEGRITY DURING LIQUID STORAGE OF GOAT SEMEN. JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES)[Internet]. 2013;6(1 (20)):19-28. Available from: https://sid.ir/paper/191753/en

IEEE: Copy

A. ABID, M. FOROZANFAR, M. HOSEINI, and M. NASRE ESFEHANI, “EVALUATION OF THE EFFECTS OF A CELL PERMEABLE ANTIOXIDANT ON MOTILITY AND MEMBRANE INTEGRITY DURING LIQUID STORAGE OF GOAT SEMEN,” JOURNAL OF ANIMAL PHYSIOLOGY AND DEVELOPMENT (QUARTERLY JOURNAL OF BIOLOGICAL SCIENCES), vol. 6, no. 1 (20), pp. 19–28, 2013, [Online]. Available: https://sid.ir/paper/191753/en

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