INVESTIGATION OF IN VITRO PRODUCTION OF HYPERICIN AND OTHER SECONDARY METABOLITS IN HYPERICUM PERFORATUM

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AZIZI MAJID; JAFARI MOFIDABADI A.; OMID BEYGI R.

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ISSN: 2588-4824

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Journal: Year:2002 | Volume:15 | Issue:1 (54 IN NATURAL RESOURCES) Page(s): 40-45

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Title

INVESTIGATION OF IN VITRO PRODUCTION OF HYPERICIN AND OTHER SECONDARY METABOLITS IN HYPERICUM PERFORATUM

Author(s)

AZIZI MAJID | JAFARI MOFIDABADI A. | OMID BEYGI R. | Issue Writer Certificate

Keywords

HYPERICUM PERTORATUM (ST. JOHNS WORT). INVITROCULTUREQ3

SECONDARY METABOLITESQ2

HYPERICINQ2

Abstract

Hypericum perforatum is an improtant medicinal plant. This plant has a great potential in phramaceutical industries. In order to produce callus, seeds of "Topas" cultivar were isolated and cultured on MS medium supplemented with 2, 4-0 (12.5 and 2.5 mg/l) and kinetin (0.1 and 1 mg/l). Callus proliferation was taken placed on the same medium for two months. Plantlets were regenerated MS medium containing BAP (2.5 and 5 mg/l) with NAA (2.5 and 5 mg/l). The results indicated high frequency of callus induction and the plantlets regeneration of this species. Increasing of BAP concentrations used higher number of plantlets. HPLC-OAO method was used to analysis SECONDARY METABOLITES of callus and in vitro plantlets. The results showed that callus with red dot on its surface, lacking HYPERICIN and pseudo HYPERICIN, while in vitro regenerated plantlets had special nodes (black dots) containing HYPERICIN and pseudohypericin. Production of other SECONDARY METABOLITES (hyperforin, Ad-hyperforin, chlorogenic acid, Iso-chlorogenic acid and apijenin) on in vitro plantlets were higher than callus.

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APA: Copy

AZIZI, MAJID, JAFARI MOFIDABADI, A., & OMID BEYGI, R.. (2002). INVESTIGATION OF IN VITRO PRODUCTION OF HYPERICIN AND OTHER SECONDARY METABOLITS IN HYPERICUM PERFORATUM. PAJOUHESH-VA-SAZANDEGI, 15(1 (54 IN NATURAL RESOURCES)), 40-45. SID. https://sid.ir/paper/19194/en

Vancouver: Copy

AZIZI MAJID, JAFARI MOFIDABADI A., OMID BEYGI R.. INVESTIGATION OF IN VITRO PRODUCTION OF HYPERICIN AND OTHER SECONDARY METABOLITS IN HYPERICUM PERFORATUM. PAJOUHESH-VA-SAZANDEGI[Internet]. 2002;15(1 (54 IN NATURAL RESOURCES)):40-45. Available from: https://sid.ir/paper/19194/en

IEEE: Copy

MAJID AZIZI, A. JAFARI MOFIDABADI, and R. OMID BEYGI, “INVESTIGATION OF IN VITRO PRODUCTION OF HYPERICIN AND OTHER SECONDARY METABOLITS IN HYPERICUM PERFORATUM,” PAJOUHESH-VA-SAZANDEGI, vol. 15, no. 1 (54 IN NATURAL RESOURCES), pp. 40–45, 2002, [Online]. Available: https://sid.ir/paper/19194/en

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