DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN CLINICAL SAMPLES AND DIFFERENTIATION FROM VACCINAL STRAIN BY RTPCR- RFLP

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GHOREYSHI S.A.; HAJIAN T.; MORSHEDI D.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Year:2003 | Volume:16 | Issue:(60 IN ANIMAL AND FISHERIES SCIENCES) Page(s): 65-69

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Title

DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN CLINICAL SAMPLES AND DIFFERENTIATION FROM VACCINAL STRAIN BY RTPCR- RFLP

Author(s)

GHOREYSHI S.A. | HAJIAN T. | MORSHEDI D. | Issue Writer Certificate

Keywords

INFECTIONS BURSAL DISEASE VIRUS. DIAGNOSIS RT-PCRQ4

Abstract

In the last two decades, endemic infectious bursal disease (IBD) have been reported worldwide. IBD is also present in Iran and heavy economic loss in poultry industry has been reported. In this study a method of RT-PCR for detection of virus in clinical samples was optimized. A 743 bp fragment of VP2 gene which is genetically variable among IBDV strains was amplified. PCR product of positive samples were digested with two restriction enzymes (RE), (Mbol and BstNI) and results were recorded. Three imported vaccine strains were also tested and results showed that RE patterns between field isolates and vaccine strains of IBDV are different. This indicates genetic variation in VP2 region of tested viruses. By using this technique, field isolates can be differentiated from vaccine strains.

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APA: Copy

GHOREYSHI, S.A., HAJIAN, T., & MORSHEDI, D.. (2003). DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN CLINICAL SAMPLES AND DIFFERENTIATION FROM VACCINAL STRAIN BY RTPCR- RFLP. PAJOUHESH-VA-SAZANDEGI, 16((60 IN ANIMAL AND FISHERIES SCIENCES)), 65-69. SID. https://sid.ir/paper/19243/en

Vancouver: Copy

GHOREYSHI S.A., HAJIAN T., MORSHEDI D.. DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN CLINICAL SAMPLES AND DIFFERENTIATION FROM VACCINAL STRAIN BY RTPCR- RFLP. PAJOUHESH-VA-SAZANDEGI[Internet]. 2003;16((60 IN ANIMAL AND FISHERIES SCIENCES)):65-69. Available from: https://sid.ir/paper/19243/en

IEEE: Copy

S.A. GHOREYSHI, T. HAJIAN, and D. MORSHEDI, “DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS IN CLINICAL SAMPLES AND DIFFERENTIATION FROM VACCINAL STRAIN BY RTPCR- RFLP,” PAJOUHESH-VA-SAZANDEGI, vol. 16, no. (60 IN ANIMAL AND FISHERIES SCIENCES), pp. 65–69, 2003, [Online]. Available: https://sid.ir/paper/19243/en

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