Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants

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Aminsalehi M.; JONOUBI P.; RAZAVI KH.; Zeynipour M.

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Journal: JOURNAL OF DEVELOPMENTAL BIOLOGY Year:2019 | Volume:11 | Issue:4 Page(s): 59-72

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Title

Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants

Author(s)

Aminsalehi M. | JONOUBI P. | RAZAVI KH. | Zeynipour M. | Issue Writer Certificate

Keywords

Anatomical structureQ1

explantQ1

in vitro cultureQ1

lateral budsQ1

RosaceaeQ2

Abstract

Rosa hybrid L. is one of the most important ornamental plants that one of the modern methods of micropropagation of Rosa hybrid Cv. Maurossia is tissue culture. In this research, after optimizing of sterilization of explants with sodium hypochlorite, MS culture medium with various concentrations of BA was used for the establishment. The highest growth rate was observed in 0. 2 mg l-1 BA. Then, explants were transferred to MS medium with different concentrations of BA, NAA and IAA. The results showed the optimal branch regenerated was obtained at 2 mg l-1 BA and 0. 5 mg l-1 IAA. In indirect organogenesis, the part of the leaf with the petioles was used and the optimum percentage was observed in 2 mg l-1 BA with 1 mgl-1 NAA. Then, explants transferred to the rooting induction medium of MS and MS/2 with IBA and Phloroglucinol. The most rooting induction was observed in MS/2 medium with 1 mg l-1 BA and 0. 2 mg l-1 Phl (Phloroglusinol). Also, due to the importance of the morphology of the Rosacea family, the anatomical studies of the vegetative organs were compared in two samples of in vivo and in vitro plants and the results showed that the general structures in both samples are similar.

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APA: Copy

Aminsalehi, M., JONOUBI, P., RAZAVI, KH., & Zeynipour, M.. (2019). Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants. JOURNAL OF DEVELOPMENTAL BIOLOGY, 11(4 ), 59-72. SID. https://sid.ir/paper/193173/en

Vancouver: Copy

Aminsalehi M., JONOUBI P., RAZAVI KH., Zeynipour M.. Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants. JOURNAL OF DEVELOPMENTAL BIOLOGY[Internet]. 2019;11(4 ):59-72. Available from: https://sid.ir/paper/193173/en

IEEE: Copy

M. Aminsalehi, P. JONOUBI, KH. RAZAVI, and M. Zeynipour, “Micro propagation of (Rosa hybrida L. cv. Maurossia) and Comparative anatomy of vegetative organs in vivo and in vitro plants,” JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 11, no. 4 , pp. 59–72, 2019, [Online]. Available: https://sid.ir/paper/193173/en

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