NITROGEN FIXATION ABILITY OF AZOSPIRILLUM SPP. ISOLATED FROM TEHRAN FOREST PARKS

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AKHAVAN SEPAHEY A.; IBRAHIMI B.; ASGHARZADE A.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2009 | Volume:1 | Issue:1 Page(s): 33-41

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Title

NITROGEN FIXATION ABILITY OF AZOSPIRILLUM SPP. ISOLATED FROM TEHRAN FOREST PARKS

Author(s)

AKHAVAN SEPAHEY A. | IBRAHIMI B. | ASGHARZADE A. | Issue Writer Certificate

Keywords

AZOSPIRILLUMQ4

MPNQ4

NITROGENASEACTIVITY AND GAS CHROMATOGRAPHYQ4

Abstract

AZOSPIRILLUM is a gram negative plump and straight rods bacterium. 4 Tehran forest parks were selected: (Chitgar, Sohanak, Sorkhehhesart, and Toska) in order to AZOSPIRILLUM isolation. Two samples are provided from each park, one of them from forested zones and the other from non forested Zone. NFb (Nitrogen Free malat) semisolid medium and RC (Rodriquez Caceres) used for culturing. The biochemical tests used for separation species. AZOSPIRILLUM species were isolated in these soils. Our researches showed three species A.brasilense, A. lipoferum and A. irakense. Microbial population is determined by MPN (Most Probably Number) method. In average, the amount of population in forested zones is 4.3×105 and in non forested zones is 1.43×105 (bacterial cell in each grams of soil (CFU/g)) by solid NFb medium. In average, the population in forested zones is 1.91×105 and in non forested zones is 0.77×105 by solid RC medium. In average, the population in planet forested zones and in non forested zones were 0.95×103 and 0.27×103 by semisolid NFb medium. Then the Nitrogen fixation ability of acquired samples was studied. In this way, by use of Rennin Media and Gas Chromatography and by transformation Acetylene to Etilen the Nitrogenase of sample were determined. Acetylene reduction in A. lipoferum was highest in comparison with other species and was 86.27 nmol/24hTube and A. irakense was least in comparison with other species and was 23.92 nmol/24hTube and A. brasilense was 56.4 nmol/24hTube.

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APA: Copy

AKHAVAN SEPAHEY, A., IBRAHIMI, B., & ASGHARZADE, A.. (2009). NITROGEN FIXATION ABILITY OF AZOSPIRILLUM SPP. ISOLATED FROM TEHRAN FOREST PARKS. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 1(1), 33-41. SID. https://sid.ir/paper/193212/en

Vancouver: Copy

AKHAVAN SEPAHEY A., IBRAHIMI B., ASGHARZADE A.. NITROGEN FIXATION ABILITY OF AZOSPIRILLUM SPP. ISOLATED FROM TEHRAN FOREST PARKS. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2009;1(1):33-41. Available from: https://sid.ir/paper/193212/en

IEEE: Copy

A. AKHAVAN SEPAHEY, B. IBRAHIMI, and A. ASGHARZADE, “NITROGEN FIXATION ABILITY OF AZOSPIRILLUM SPP. ISOLATED FROM TEHRAN FOREST PARKS,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 1, no. 1, pp. 33–41, 2009, [Online]. Available: https://sid.ir/paper/193212/en

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