DETERMINATION OF CANDIDA SPECIES IN ACUTE AND RECURRENT CANDIDA VULVOVAGINITIS

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PANAHI F.; KORDBACHEH P.; REZAIE S.; ZEINI F.; ZERAATI H.; SAFARA M.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2009 | Volume:1 | Issue:3 Page(s): 7-12

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Title

DETERMINATION OF CANDIDA SPECIES IN ACUTE AND RECURRENT CANDIDA VULVOVAGINITIS

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Keywords

CANDIDAQ4

VAGINITISQ4

CANDIDIASISQ4

CHROM AGARQ4

Abstract

The incidence of CANDIDA vulvovaginitis caused by non-albicans CANDIDA species is dramatically rising. Most non-albicans species have higher azole MICs and infections caused by them are often difficult to treat. Accurate diagnosis depends on cultures methods that will yield correct identification of CANDIDA species. Vaginal discharges were obtained from 122 women with acute (n=61) and recurrent (n=61) CANDIDA vulvovagintis. All specimens were inoculated on CHROM AGAR medium to identify CANDIDA species and detection of mixed infection. In order to confirm the diagnosis, all isolates also plated on cornmeal agar and identified by carbohydrate assimilation using API20C cards.A total of 127 isolates were obtained from 122 vaginal specimens cultured on CHROM AGAR medium. 5 patients had mixed infection with two species. CANDIDA albicans was the most common isolated species from acute (82.7%) and recurrent (57.8%) patient groups and the most common non-albicans species identified by this method was CANDIDA krusei. Cornmeal agar test showed that 22.2% and 45.3% of the isolates were non-albicans species in acute and recurrent cases respectively. But API20C test revealed that CANDIDA glabrata was the most common non-albicans species isolated in this study.Infection with non-albicans CANDIDA species is more common in recurrent CANDIDA vulvovaginitis. CHROM AGAR is a useful medium in detection of mixed CANDIDA infection. But in spite of its ability in identifying CANDIDA albicans and non-albicans species in some situation, this medium is not reliable for definite diagnosis of CANDIDA species.

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APA: Copy

PANAHI, F., KORDBACHEH, P., REZAIE, S., ZEINI, F., ZERAATI, H., & SAFARA, M.. (2009). DETERMINATION OF CANDIDA SPECIES IN ACUTE AND RECURRENT CANDIDA VULVOVAGINITIS. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 1(3), 7-12. SID. https://sid.ir/paper/193220/en

Vancouver: Copy

PANAHI F., KORDBACHEH P., REZAIE S., ZEINI F., ZERAATI H., SAFARA M.. DETERMINATION OF CANDIDA SPECIES IN ACUTE AND RECURRENT CANDIDA VULVOVAGINITIS. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2009;1(3):7-12. Available from: https://sid.ir/paper/193220/en

IEEE: Copy

F. PANAHI, P. KORDBACHEH, S. REZAIE, F. ZEINI, H. ZERAATI, and M. SAFARA, “DETERMINATION OF CANDIDA SPECIES IN ACUTE AND RECURRENT CANDIDA VULVOVAGINITIS,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 1, no. 3, pp. 7–12, 2009, [Online]. Available: https://sid.ir/paper/193220/en

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