Investigation of polymorphism of DGAT1 gene in Iranian buffaloes

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Naserkheil Masoumeh; Miraie Ashtiani Seyed Reza; SADEGHI MOSTAFA; NEJATI JAVAREMI ARDESHIR; Lee Deukhwan

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Animal Science Year:2016 | Volume:47 | Issue:2 Page(s): 175-183

Download Full-Text

Persian Verion

View:

1,969

Download:

Cites:

Information Journal Paper

Title

Investigation of polymorphism of DGAT1 gene in Iranian buffaloes

Author(s)

Keywords

DGAT1 geneQ2

Iranian buffaloQ2

sequence analysisQ2

SNPsQ2

Abstract

Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. In this research, the polymorphism of the region spanning exon 3 to exon 17 of the DGAT1 gene of three indigenous Iranian buffalo populations was investigated. A total number of samples of 200 buffaloes (Khuzestani, Shomali and Azari) were collected to extract DNA and the desired fragments were amplified by polymerase chain reaction (PCR). Genotyping was performed using direct sequencing and analyzed by BioEdit software. sequence analysis showed novel SNPs in comparative to the reference sequence GenBank (DQ886485) at nucleotide positions g. 6097A>G, g. 7036C>T, g. 7338G>A, g. 7710C>T, g. 8087C>T, g. 8259G>A, g. 8275G>A and g. 8426C>T of DGAT1 gene in buffalo populations. A comparison with Indian buffalo revealed three exonic SNPs, one of which was non-synonymous. A Unique 22 base insertion has been observed in the intron 10. The results described here genetic diversity and could be useful in genetic and breeding programs in Iranian buffalo.

Cites

No record.

References

No record.

Cite

APA: Copy

Naserkheil, Masoumeh, Miraie Ashtiani, Seyed Reza, SADEGHI, MOSTAFA, NEJATI JAVAREMI, ARDESHIR, & Lee, Deukhwan. (2016). Investigation of polymorphism of DGAT1 gene in Iranian buffaloes. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 47(2 ), 175-183. SID. https://sid.ir/paper/193559/en

Vancouver: Copy

Naserkheil Masoumeh, Miraie Ashtiani Seyed Reza, SADEGHI MOSTAFA, NEJATI JAVAREMI ARDESHIR, Lee Deukhwan. Investigation of polymorphism of DGAT1 gene in Iranian buffaloes. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2016;47(2 ):175-183. Available from: https://sid.ir/paper/193559/en

IEEE: Copy

Masoumeh Naserkheil, Seyed Reza Miraie Ashtiani, MOSTAFA SADEGHI, ARDESHIR NEJATI JAVAREMI, and Deukhwan Lee, “Investigation of polymorphism of DGAT1 gene in Iranian buffaloes,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 47, no. 2 , pp. 175–183, 2016, [Online]. Available: https://sid.ir/paper/193559/en

Related Journal Papers