EFFECT OF ADDING L-GLUTAMINE TO TRIS DILUENT ON CRYOPRESERVATION OF COATED SPERM OF TALESHI RAM

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HASANPOOR SANAZ; ROOSTAEI ALI MEHR MOHAMMAD; MOHAMMADI MEHRDAD

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Journal: Iranian Journal of Animal Science Year:2014 | Volume:45 | Issue:3 Page(s): 289-296

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Title

EFFECT OF ADDING L-GLUTAMINE TO TRIS DILUENT ON CRYOPRESERVATION OF COATED SPERM OF TALESHI RAM

Author(s)

HASANPOOR SANAZ | ROOSTAEI ALI MEHR MOHAMMAD | MOHAMMADI MEHRDAD | Issue Writer Certificate

Keywords

L-GLUTAMINEQ2

TALESHI RAMQ2

COATED SPERMATOZOAQ1

FREEZINGQ2

Abstract

Experiment was conducted to evaluate the effect of L-GLUTAMINE on coated sperm FREEZING by using four rams. Semen was collected by artificial vagina contact with a tube containing Tris-fructose-egg yolk 15%. Samples were pooled and centrifuged. After removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mM L-GLUTAMINE. Samples were frozen by using liquid nitrogen After two weeks, samples were thawed and incubated at 37 °C for nine h. Sperm motility, plasma membrane integrity, viability (Hoechst 33258) and acrosome reaction (PNA-Alexa -flur-488) were investigated at 0, 3, 6 and 9 h. The main effect of L-GLUTAMINE showed that sperm viability was higher in 0 (35.95%), 40 (40.65%) and 80 (45.95%) mM L-GLUTAMINE than 160 mM L-GLUTAMINE (30.40%; P<0.05). There was an interaction between incubation time and L-GLUTAMINE on sperm motility and plasma membrane integrity (P<0.05). After thawing and 3 h later, the highest of plasma membrane integrity was observed in 80 mM L-GLUTAMINE (49.25% and 47.34% respectively; P<0.05). Nine hours after thawing, the highest of sperm motility (26.4%) was observed in 80 mM L-GLUTAMINE (P<0.05). Therefore, the addition of 80 mM L-GLUTAMINE to Tris-glycerol diluent (5%) improves coated sperm survival.

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APA: Copy

HASANPOOR, SANAZ, ROOSTAEI ALI MEHR, MOHAMMAD, & MOHAMMADI, MEHRDAD. (2014). EFFECT OF ADDING L-GLUTAMINE TO TRIS DILUENT ON CRYOPRESERVATION OF COATED SPERM OF TALESHI RAM. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 45(3), 289-296. SID. https://sid.ir/paper/193619/en

Vancouver: Copy

HASANPOOR SANAZ, ROOSTAEI ALI MEHR MOHAMMAD, MOHAMMADI MEHRDAD. EFFECT OF ADDING L-GLUTAMINE TO TRIS DILUENT ON CRYOPRESERVATION OF COATED SPERM OF TALESHI RAM. IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2014;45(3):289-296. Available from: https://sid.ir/paper/193619/en

IEEE: Copy

SANAZ HASANPOOR, MOHAMMAD ROOSTAEI ALI MEHR, and MEHRDAD MOHAMMADI, “EFFECT OF ADDING L-GLUTAMINE TO TRIS DILUENT ON CRYOPRESERVATION OF COATED SPERM OF TALESHI RAM,” IRANIAN JOURNAL OF ANIMAL SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 45, no. 3, pp. 289–296, 2014, [Online]. Available: https://sid.ir/paper/193619/en

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