THE DETECTION OF FIMBRIAL ENCODING GENES INE. COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION

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NAZEMI A.; NADERI M.; JAFARPOUR M.; MIRI NARGESI M.S.; SHARIFI SH.

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Journal: MEDICAL LABORATORY JOURNAL Year:2011 | Volume:4 | Issue:2 Page(s): 31-37

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Title

THE DETECTION OF FIMBRIAL ENCODING GENES INE. COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION

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Keywords

URINARY TRACT INFECTIONQ3

FIMBRIAQ3

BACTERIALQ2

ESCHERICHIA COLI (UPEC)Q2

Abstract

Bachground and objectives: The ability of adherence to the surface of host cell is very critical in the colonization of microbial pathogens. It has been revealed that E.coli strains that infect urinary tracts have different FIMBRIA such as I, S, P, FIC, Dr, and FIMBRIAl adhesions.Material and Methods: In this study, 363 urine samples were obtained from patients with URINARY TRACT INFECTIONs referred to clinical laboratories in Western areas of Tehran, 2008-2010. By using biochemical tests, 200 samples were confirmed to be E.coli. First, DNA was extracted by boiling method and then the presence of FIMBRIA fim, sfa, pap, foc, and afa genes tested by PCR.Results: of 200 samples, the frequency of FIMBRIA fim, sfa, pap, foc, and afa genes are 188 (%94), 34 (%17), 20 (%10), 61 (%31) and 71 (%35.5), respectively.Conclusion: The results show that fim and sfa are the most FIMBRIAl genes of E.coliisolated from urine samples. This information can be valuable in etiology of URINARY TRACT INFECTION (UTI), UTI administration, and vaccine production.

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مقایسه فراوانی ژن های vat1 وfimC درجدایه های اشریشیاکولی بافتی مبتلایان به سرطان کولورکتال و بیماری التهابی روده بزرگ در جمعیت ایرانی

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APA: Copy

NAZEMI, A., NADERI, M., JAFARPOUR, M., MIRI NARGESI, M.S., & SHARIFI, SH.. (2011). THE DETECTION OF FIMBRIAL ENCODING GENES INE. COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION. MEDICAL LABORATORY JOURNAL, 4(2), 31-37. SID. https://sid.ir/paper/194755/en

Vancouver: Copy

NAZEMI A., NADERI M., JAFARPOUR M., MIRI NARGESI M.S., SHARIFI SH.. THE DETECTION OF FIMBRIAL ENCODING GENES INE. COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION. MEDICAL LABORATORY JOURNAL[Internet]. 2011;4(2):31-37. Available from: https://sid.ir/paper/194755/en

IEEE: Copy

A. NAZEMI, M. NADERI, M. JAFARPOUR, M.S. MIRI NARGESI, and SH. SHARIFI, “THE DETECTION OF FIMBRIAL ENCODING GENES INE. COLI STRAINS ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION,” MEDICAL LABORATORY JOURNAL, vol. 4, no. 2, pp. 31–37, 2011, [Online]. Available: https://sid.ir/paper/194755/en

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