RAPID DNA EXTRACTION OF BACTERIAL GENOME OF STAPHYLOCOCCUS AUREUS USING LAUNDRY DETERGENTS AND ASSESSMENT OF THE EFFICIENCY OF DNA IN DOWNSTREAM PROCESS USING PCR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOUSAZADE MOGHADAM M.; BABAVALIAN H.; MIRNEJAD R.; SHAKERI F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MEDICAL LABORATORY JOURNAL Year:2012 | Volume:6 | Issue:1 Page(s): 35-42

Download Full-Text

Persian Verion

View:

2,841

Download:

Cites:

Information Journal Paper

Title

RAPID DNA EXTRACTION OF BACTERIAL GENOME OF STAPHYLOCOCCUS AUREUS USING LAUNDRY DETERGENTS AND ASSESSMENT OF THE EFFICIENCY OF DNA IN DOWNSTREAM PROCESS USING PCR

Author(s)

MOUSAZADE MOGHADAM M. | BABAVALIAN H. | MIRNEJAD R. | SHAKERI F. | Issue Writer Certificate

Keywords

BACTERIAL GENOMEQ2

DNA EXTRACTIONQ2

LAUNDRY POWDERQ2

PCRQ2

STAPHYLOCOCCUS AUREUSQ2

Abstract

Background and objectives: Genomic DNA EXTRACTION of bacterial cells is of processes performed normally in most biological laboratories; therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA EXTRACTION of STAPHYLOCOCCUS AUREUS was investigated using some laundry detergent brands available in Iran to achieve a rapid and cost effective method.Material and Methods: five-enzyme Taj brand, three-enzyme Saftlan brand, and Darya and Pak brands without enzyme were used in the concentrations of 10, 20, 40, 80 mg/L. Afterwards, in order to evaluate the efficiency of extracted DNA in downstream processing, PCR test was performed for femA gene in the genome of STAPHYLOCOCCUS AUREUS.Results: DNA EXTRACTION using different concentrations of the brands show that extracted DNA using 40 mg/L Saftlan and Taj brand powders have the best results according concentration (mg/ml) and purity (A260/A280) parameters. These parameters are 387.5; 1.88 (Taj), 254.1; 2.80 (Softlan), 396.6; 1.95 (Manual) and 423.3; 2.2 (Kit), respectively. Afterward, the PCR test results by show that DNA EXTRACTION using laundry detergents has no effect on its efficiency in order to be used in downstream processes.Conclusion: These results indicate that the proper concentrations of laundry detergents can be used to extract genomic DNA with similar efficiency to kit and manual extraction methods.

Cites

No record.

References

No record.

Cite

APA: Copy

MOUSAZADE MOGHADAM, M., BABAVALIAN, H., MIRNEJAD, R., & SHAKERI, F.. (2012). RAPID DNA EXTRACTION OF BACTERIAL GENOME OF STAPHYLOCOCCUS AUREUS USING LAUNDRY DETERGENTS AND ASSESSMENT OF THE EFFICIENCY OF DNA IN DOWNSTREAM PROCESS USING PCR. MEDICAL LABORATORY JOURNAL, 6(1), 35-42. SID. https://sid.ir/paper/194784/en

Vancouver: Copy

MOUSAZADE MOGHADAM M., BABAVALIAN H., MIRNEJAD R., SHAKERI F.. RAPID DNA EXTRACTION OF BACTERIAL GENOME OF STAPHYLOCOCCUS AUREUS USING LAUNDRY DETERGENTS AND ASSESSMENT OF THE EFFICIENCY OF DNA IN DOWNSTREAM PROCESS USING PCR. MEDICAL LABORATORY JOURNAL[Internet]. 2012;6(1):35-42. Available from: https://sid.ir/paper/194784/en

IEEE: Copy

M. MOUSAZADE MOGHADAM, H. BABAVALIAN, R. MIRNEJAD, and F. SHAKERI, “RAPID DNA EXTRACTION OF BACTERIAL GENOME OF STAPHYLOCOCCUS AUREUS USING LAUNDRY DETERGENTS AND ASSESSMENT OF THE EFFICIENCY OF DNA IN DOWNSTREAM PROCESS USING PCR,” MEDICAL LABORATORY JOURNAL, vol. 6, no. 1, pp. 35–42, 2012, [Online]. Available: https://sid.ir/paper/194784/en

Related Journal Papers