THE COMPARISON OF PCR TECHNIQUE AND API-20E KIT WITH THE CONVENTIONAL BIOMEDICAL METHODS FOR THE IDENTIFICATION OF SALMONELLA SPECIES IN LABORATORY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLTAN DALLAL M.M.; RAHIMI FORUSHANI A.; SEDIGH MAROUFI SH.; SHARIFI YAZDI M.K.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MEDICAL LABORATORY JOURNAL Year:2012 | Volume:5 | Issue:2 Page(s): 20-27

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

3,803

Download:

Cites:

Information Journal Paper

Title

THE COMPARISON OF PCR TECHNIQUE AND API-20E KIT WITH THE CONVENTIONAL BIOMEDICAL METHODS FOR THE IDENTIFICATION OF SALMONELLA SPECIES IN LABORATORY

Author(s)

SOLTAN DALLAL M.M. | RAHIMI FORUSHANI A. | SEDIGH MAROUFI SH. | SHARIFI YAZDI M.K. | Issue Writer Certificate

Keywords

SALMONELLAQ3

CONVENTIONAL IDENTIFICATIONQ3

MOLECULAR IDENTIFICATIONQ2

Abstract

Background and objectives: SALMONELLA is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to treatment failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of SALMONELLA.Material and Methods: In this study, 470 specimens were taken from children, with acute gastroenteritis, referred to teaching hospitals called Imam, Shariati and children medical centre. The specimens were transferred to microbiology laboratory in public health school for identification of SALMONELLA with PCR and API-20E methods.Results: Of 470 specimens, 65 (13.8%) are positive for SALMONELLA in hospital laboratory, while 37 (7.9%) for API-20E and 39 (8.3%) for PCR are positive. The results of antibiotic sensitivity tests on 39 SALMONELLA isolated from diarrhea specimens show that 73.3% of them are resistance to at least one of the sixteen antibiotics tested.Conclusion: Based on the the results, there is significant difference (P<0.05) between conventional method, API-20E and PCR.

Cites

References

No record.

Cite

APA: Copy

SOLTAN DALLAL, M.M., RAHIMI FORUSHANI, A., SEDIGH MAROUFI, SH., & SHARIFI YAZDI, M.K.. (2012). THE COMPARISON OF PCR TECHNIQUE AND API-20E KIT WITH THE CONVENTIONAL BIOMEDICAL METHODS FOR THE IDENTIFICATION OF SALMONELLA SPECIES IN LABORATORY. MEDICAL LABORATORY JOURNAL, 5(2), 20-27. SID. https://sid.ir/paper/194805/en

Vancouver: Copy

SOLTAN DALLAL M.M., RAHIMI FORUSHANI A., SEDIGH MAROUFI SH., SHARIFI YAZDI M.K.. THE COMPARISON OF PCR TECHNIQUE AND API-20E KIT WITH THE CONVENTIONAL BIOMEDICAL METHODS FOR THE IDENTIFICATION OF SALMONELLA SPECIES IN LABORATORY. MEDICAL LABORATORY JOURNAL[Internet]. 2012;5(2):20-27. Available from: https://sid.ir/paper/194805/en

IEEE: Copy

M.M. SOLTAN DALLAL, A. RAHIMI FORUSHANI, SH. SEDIGH MAROUFI, and M.K. SHARIFI YAZDI, “THE COMPARISON OF PCR TECHNIQUE AND API-20E KIT WITH THE CONVENTIONAL BIOMEDICAL METHODS FOR THE IDENTIFICATION OF SALMONELLA SPECIES IN LABORATORY,” MEDICAL LABORATORY JOURNAL, vol. 5, no. 2, pp. 20–27, 2012, [Online]. Available: https://sid.ir/paper/194805/en

Related Journal Papers